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Titolo:
Liquid transport properties of porcine tracheal epithelium
Autore:
Crews, A; Taylor, AE; Ballard, ST;
Indirizzi:
Univ S Alabama, Coll Med, Dept Physiol, MSB 3024, Mobile, AL 36688 USA Univ S Alabama Mobile AL USA 36688 hysiol, MSB 3024, Mobile, AL 36688 USA
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 2, volume: 91, anno: 2001,
pagine: 797 - 802
SICI:
8750-7587(200108)91:2<797:LTPOPT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
AIRWAY SURFACE LIQUID; CYSTIC-FIBROSIS; FLUID TRANSPORT; WATER TRANSPORT; CHLORIDE; GLANDS;
Keywords:
fluid transport; cystic fibrosis; cystic fibrosis transmembrane conductance regulator protein; pig trachea; chloride channels;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Ballard, ST Univ S Alabama, Coll Med, Dept Physiol, MSB 3024, Mobile, AL 36688 USA Univ S Alabama Mobile AL USA 36688 3024, Mobile, AL 36688 USA
Citazione:
A. Crews et al., "Liquid transport properties of porcine tracheal epithelium", J APP PHYSL, 91(2), 2001, pp. 797-802

Abstract

Because of its possible importance to the etiology of cystic fibrosis lungdisease, the ion and water transport properties of tracheal epithelium were studied. Net liquid flux (J(V)) across porcine tracheal epithelium was measured in vitro using blue dextran as a volume probe. Luminal instillation of isosmotic sucrose solution (280 mM) induced a small net secretion of liquid (7.0 +/-1.7 nl.cm(-2).s(-1)), whereas luminal hyposmotic sucrose solutions (220 or 100 mM) induced substantial and significant (p < 0.05) liquid absorption (34.5<plus/minus>12 and 38.1 +/-7.3 nl.cm(-2).s(-1), respectively). When the luminal solution was normal (isosmotic) Krebs buffer, liquid was absorbed at 10.2 +/-1.1 nl.cm(-2).s(-1). Absorptive JV was abolished by 100 mM amiloride in the luminal solution and significantly reduced when the luminal solution was Na+-free Krebs solution. Absorptive JV was not significantly affected by 300 muM 5-nitro-2-(3-phenylpropylamino) benzoate or 100 muM diphenylamine-2-carboxylic acid, both cystic fibrosis transmembrane conductance regulator protein (CFTR) inhibitors, in the instillate but was significantly reduced by 60% when the luminal solution was Cl--free Krebs solution. We conclude that water freely permeates porcine tracheal epithelium and that absorption of liquid is normally driven by active transcellular Natransport and does not require the CFTR.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:25:33