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Titolo:
Extracellular glutathione inhibits oxygen-induced permeability changes in alveolar epithelial monolayers
Autore:
Roum, JH; Aledia, AS; Carungcong, LA; Kim, KJ; Borok, Z;
Indirizzi:
Univ Calif Irvine, Med Ctr, Dept Med, Orange, CA 92868 USA Univ Calif Irvine Orange CA USA 92868 Ctr, Dept Med, Orange, CA 92868 USA Univ So Calif, Will Rogers Inst, Pulm Res Ctr, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 Res Ctr, Los Angeles, CA 90033 USA Univ So Calif, Dept Med, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 ept Med, Los Angeles, CA 90033 USA Univ So Calif, Dept Physiol & Biophys, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 Biophys, Los Angeles, CA 90033 USA Univ So Calif, Dept Biomed Engn, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 ed Engn, Los Angeles, CA 90033 USA Univ So Calif, Dept Mol Pharmacol & Toxicol, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 Toxicol, Los Angeles, CA 90033 USA
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 2, volume: 91, anno: 2001,
pagine: 748 - 754
SICI:
8750-7587(200108)91:2<748:EGIOPC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOWER RESPIRATORY-TRACT; IDIOPATHIC PULMONARY FIBROSIS; ACTIVE SODIUM-TRANSPORT; HYPEROXIC LUNG INJURY; SPRAGUE-DAWLEY RATS; PRETERM GUINEA-PIG; IN-VITRO; II PNEUMOCYTES; LINING FLUID; INTRACELLULAR GLUTATHIONE;
Keywords:
hyperoxia; rat; oxidant; antioxidant; glutathione reductase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Roum, JH Univ Calif Irvine, Med Ctr, Dept Med, Bldg 53,Rm 119,101 City Dr S, Orange, CA 92868 USA Univ Calif Irvine Bldg 53,Rm 119,101 City Dr S Orange CA USA 92868
Citazione:
J.H. Roum et al., "Extracellular glutathione inhibits oxygen-induced permeability changes in alveolar epithelial monolayers", J APP PHYSL, 91(2), 2001, pp. 748-754

Abstract

Exposure to high fractional inspired oxygen for 24 h increases permeability of the alveolar epithelium, contributing to the clinical manifestations of oxygen toxicity. Utilizing a model of the alveolar epithelium in which isolated rat type II cells form polarized monolayers on polycarbonate filters[transepithelial resistance (R-t) > 1 k Omega .cm(2) by day 4], we evaluated the ability of reduced glutathione (GSH) to ameliorate these changes. Onday 4, apical fluid was replaced with culture medium containing 1) no additives, 2) GSH (500 muM), or 3) GSH (500 muM) + glutathione reductase (0.5 U/ml) + nicotinamide adenine dinucleotide phosphate (250 muM). Monolayers were exposed (for 24 h) to room air (control) or 95% O-2, each containing 5% CO2. After 24 h of hyperoxia, R-t for condition 1 decreased by 45% comparedwith control (P < 0.001). In conditions 2 and 3, R-t did not decrease significantly (P = not significant). Hyperoxia-induced decreases in active ion transport were observed for conditions 1 and 2 (P < 0.05), but not for condition 3 (P = not significant). These findings indicate that extracellular GSH may protect the alveolar epithelium against hyperoxia-induced injury. Addition of glutathione reductase and nicotinamide adenine dinucleotide phosphate may further augment these protective effects of GSH.

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Documento generato il 01/04/20 alle ore 11:33:00