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Titolo:
Insight into skeletal muscle mechanotransduction: MAPK activation is quantitatively related to tension
Autore:
Martineau, LC; Gardiner, PF;
Indirizzi:
Univ Montreal, Dept Kinesiol, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 ol, Montreal, PQ H3C 3J7, Canada
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 2, volume: 91, anno: 2001,
pagine: 693 - 702
SICI:
8750-7587(200108)91:2<693:IISMMM>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOCAL ADHESION KINASE; VASCULAR SMOOTH-MUSCLE; PROTEIN-KINASE; SIGNALING PATHWAYS; C-JUN; ENDOTHELIAL-CELLS; CARDIAC MYOCYTES; GENE-EXPRESSION; CYCLIC STRAIN; STRETCH;
Keywords:
intracellular signaling; mitogen-activated protein kinases; c-Jun NH2-terminal kinase; eccentric contraction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Gardiner, PF Univ Montreal, Dept Kinesiol, POB 6128 Ctrville, Montreal, PQH3C 3J7, Canada Univ Montreal POB 6128 Ctrville Montreal PQ Canada H3C 3J7da
Citazione:
L.C. Martineau e P.F. Gardiner, "Insight into skeletal muscle mechanotransduction: MAPK activation is quantitatively related to tension", J APP PHYSL, 91(2), 2001, pp. 693-702

Abstract

The mechanism by which mechanical forces acting through skeletal muscle cells generate intracellular signaling, known as mechanotransduction, and thedetails of how gene expression and cell size are regulated by this signaling are poorly understood. Mitogen-activated protein kinases (MAPKs) are known to be involved in mechanically induced signaling in various cell types, including skeletal muscle where MAPK activation has been reported in response to contraction and passive stretch. Therefore, the investigation of MAPKactivation in response to mechanical stress in skeletal muscle may yield important information about the mechanotransduction process. With the use ofa rat plantaris in situ preparation, a wide range of peak tensions was generated through passive stretch and concentric, isometric, and eccentric contractile protocols, and the resulting phosphorylation of c-Jun NH2-terminalkinase (JNK), extracellular regulated kinase (ERK), and p38 MAPKs was assessed. Isoforms of JNK and ERK MAPKs were found to be phosphorylated in a tension-dependent manner, such that eccentric. isometric. concentric. passivestretch. Peak tension was found to be a better predictor of MAPK phosphorylation than time-tension integral or rate of tension development. Differences in maximal response amplitude and sensitivity between JNK and ERK MAPKs suggest different roles for these two kinase families in mechanically induced signaling. A strong linear relationship between p54 JNK phosphorylation and peak tension over a 15-fold range in tension (r(2) = 0.89, n = 32) was observed, supporting the fact that contraction-type differences can be explained in terms of tension and demonstrating that MAPK activation is a quantitative reflection of the magnitude of mechanical stress applied to muscle. Thus the measurement of MAPK activation, as an assay of skeletal muscle mechanotransduction, may help elucidate mechanically induced hypertrophy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/06/20 alle ore 23:41:56