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Titolo:
Real-time PCR used to measure stress-induced changes in the expression of the genes of the alginate pathway of Pseudomonas aeruginosa
Autore:
Edwards, KJ; Saunders, NA;
Indirizzi:
Cent Publ Hlth Lab, Virus Reference Div, Mol Biol Unit, London NW9 5HT, England Cent Publ Hlth Lab London England NW9 5HT Unit, London NW9 5HT, England
Titolo Testata:
JOURNAL OF APPLIED MICROBIOLOGY
fascicolo: 1, volume: 91, anno: 2001,
pagine: 29 - 37
SICI:
1364-5072(200107)91:1<29:RPUTMS>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYSTIC-FIBROSIS PATIENTS; MUCOIDY; CONVERSION; DIFFERENTIATION; PATHOGENESIS; REGULATORS; INDUCTION; MEMBER; ALGR; MUCB;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Saunders, NA Cent Publ Hlth Lab, Virus Reference Div, Mol Biol Unit, 61 Colindale Ave, London NW9 5HT, England Cent Publ Hlth Lab 61 Colindale Ave London England NW9 5HT d
Citazione:
K.J. Edwards e N.A. Saunders, "Real-time PCR used to measure stress-induced changes in the expression of the genes of the alginate pathway of Pseudomonas aeruginosa", J APPL MICR, 91(1), 2001, pp. 29-37

Abstract

Aims: To measure the concentration of mRNAs transcribed from four genes involved in alginate production using real-time PCR. Methods and Results: The mRNA concentrations in cells grown in normal and stress conditions were compared. A difference in the expression of algD, the key gene leading to overproduction of alginate, was detected between alginate-producing and non-alginate-producing strains grown under normal conditions. After growth on 3% ethanol (known to stimulate alginate production), but not after heat-shock, an increase in algD mRNA levels and a corresponding decrease in mucB (a regulatory gene) mRNA levels were detected in all strains. Conclusions: The quantitative results suggest that the mucB gene may have a role in recognition of stress conditions, and that having a disrupted mucA gene does not always result in a mucoid phenotype. Significance and Impact of the Study: Real-time PCR can be used to quantify mRNA and is a convenient method of analysing bacterial gene expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 11:34:29