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Titolo:
Molecular cloning and characterization of human WNT5B on chromosome 12p13.3 region
Autore:
Saitoh, T; Katoh, M;
Indirizzi:
Natl Canc Ctr, Res Inst, Div Genet, Genet & Cell Biol Sect,Chuo Ku, Tokyo 1040045, Japan Natl Canc Ctr Tokyo Japan 1040045 iol Sect,Chuo Ku, Tokyo 1040045, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 2, volume: 19, anno: 2001,
pagine: 347 - 351
SICI:
1019-6439(200108)19:2<347:MCACOH>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
XENOPUS EMBRYOS; BETA-CATENIN; CANCER; GENE; MUTATIONS; AXIS; EXPRESSION; WNT-5A; MEMBER; FAMILY;
Keywords:
WNT; frizzled; gastric cancer; beta-catenin; TCF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Katoh, M Natl Canc Ctr, Res Inst, Div Genet, Genet & Cell Biol Sect,Chuo Ku, Tsukiji 5 Chome, Tokyo 1040045, Japan Natl Canc Ctr Tsukiji 5 Chome Tokyo Japan 1040045 1040045, Japan
Citazione:
T. Saitoh e M. Katoh, "Molecular cloning and characterization of human WNT5B on chromosome 12p13.3 region", INT J ONCOL, 19(2), 2001, pp. 347-351

Abstract

WNT signaling pathway plays a key role in carcinogenesis and embryogenesis. We have cloned and characterized the human WNT5B. Overlapping WNT5B cDNAs, containing 1080-bp ORF, were isolated. WNT5B encoded a 359-amino-acid polypeptide with the N-terminal signal peptide, four N-linked glycosylation sites, and consensus amino-acid residues conserved among the WNT family. WNT5B showed 80.5% total-amino-acid identity with WNT5A. Comparison between nucleotide sequence of WNT5B cDNA and human genome draft sequences revealed that the WNT5B gene, consisting of 4 exons, was located on human chromosome 12p13.3 region. Northern blot analyses with W5B2 probe detected the 2.8- and2.4-kb WNT5B mRNAs. WNT5B was moderately expressed in adult prostate and fetal brain, and weakly expressed in fetal lung, kidney, adult liver, ovary,and small intestine. Among human cancer cell lines, WNT5B was expressed ingastric cancer cell lines MKN7, MKN45, KATO-III, and a teratocarcinoma cell line NT2. WNT5B might be implicated in human carcinogenesis through activation of the WNT-beta -catenin-TCF signaling pathway, just like Wnt5a.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 11:46:13