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Titolo:
Assessment by D-[H-3]mannoheptulose uptake of B-cell density in isolated pancreatic islets from Goto-Kakizaki rats
Autore:
Sener, A; Ladriere, L; Malaisse, WJ;
Indirizzi:
Free Univ Brussels, Expt Med Lab, B-1070 Brussels, Belgium Free Univ Brussels Brussels Belgium B-1070 Lab, B-1070 Brussels, Belgium
Titolo Testata:
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
fascicolo: 2, volume: 8, anno: 2001,
pagine: 177 - 180
SICI:
1107-3756(200108)8:2<177:ABDUOB>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE TRANSPORTERS; INSULIN; EXPRESSION; GLYCOLYSIS; SECRETION; LEUCINE; NIDDM;
Keywords:
D-[H-3]mannoheptulose; pancreatic islets; GK rats;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Malaisse, WJ Free Univ Brussels, Expt Med Lab, 808 Route Lennik, B-1070 Brussels, Belgium Free Univ Brussels 808 Route Lennik Brussels Belgium B-1070
Citazione:
A. Sener et al., "Assessment by D-[H-3]mannoheptulose uptake of B-cell density in isolated pancreatic islets from Goto-Kakizaki rats", INT J MOL M, 8(2), 2001, pp. 177-180

Abstract

The uptake of D-[H-3]mannoheptulose by isolated pancreatic islets was recently proposed as a tool to assess the relative contribution of insulin-producing cells to the total mass of the islets. In the present study, the uptake of the tritiated heptose over 60 min incubation at 37 degreesC was about21% lower in islets from hereditarily diabetic rats (GK rats) than in islets from control animals, this decrease being virtually identical to that documented previously by morphometric analysis of islets From the same type of rats. The intracellular (HOH)-H-3 space and extracellular [U-C-14]sucrosespace were not significantly different in control and diabetic rats, at least when the comparison was restricted to animals of the same sex. There was a trend, however, towards a somewhat lower D-[5-H-3]-glucose intracellular distribution space in islets from GK rats, as compared to control animals. These findings provide further support to the validity of D-[H-3]mannoheptulose uptake as a tool to assess the density of insulin-producing cells inisolated islets.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 10:50:35