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Titolo:
Mesoderm formation by isolated and cultivated 8-cell stage blastomeres of the teleost, Leucopsarion ptersii (shiro-uo)
Autore:
Saito, T; Kamimoto, M; Miyake, A; Yamaha, E; Suzuki, T; Nakatsuji, N; Nakatsuji, T;
Indirizzi:
Tokai Univ, Dept Marine Biol, Grad Sch Marine Sci & Technol, Shimizu, Shizuoka 424, Japan Tokai Univ Shimizu Shizuoka Japan 424 chnol, Shimizu, Shizuoka 424, Japan Hokkaido Univ, Fac Fisheries, Nanae Fish Culture Expt Stn, Hakodate, Hokkaido 041, Japan Hokkaido Univ Hakodate Hokkaido Japan 041 , Hakodate, Hokkaido 041, Japan Natl Res Inst Aquaculture, Metab Sect, Nansei, Mie 51601, Japan Natl Res Inst Aquaculture Nansei Mie Japan 51601 Nansei, Mie 51601, Japan Kyoto Univ, Inst Frontier Med Sci, Kyoto, Japan Kyoto Univ Kyoto JapanKyoto Univ, Inst Frontier Med Sci, Kyoto, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
fascicolo: 4, volume: 45, anno: 2001,
pagine: 661 - 668
SICI:
0214-6282(200106)45:4<661:MFBIAC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ERYTHROID-DIFFERENTIATION FACTOR; EARLY XENOPUS-EMBRYOS; YOLK SYNCYTIAL LAYER; DORSAL DETERMINANTS; ZEBRAFISH EMBRYOS; CARASSIUS-AURATUS; PATTERN-FORMATION; NIEUWKOOP-CENTER; ACTIVIN-A; EXPRESSION;
Keywords:
ice goby (shiro-uo); 8-cell stage; isolated blastomeres; mesoderm induction; teleost embryogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Nakatsuji, T Tokai Univ, Sch Marine Sci & Technol, 3-20-1 Orido, Shimizu, Shizuoka 4248610, Japan Tokai Univ 3-20-1 Orido Shimizu Shizuoka Japan 4248610 Japan
Citazione:
T. Saito et al., "Mesoderm formation by isolated and cultivated 8-cell stage blastomeres of the teleost, Leucopsarion ptersii (shiro-uo)", INT J DEV B, 45(4), 2001, pp. 661-668

Abstract

Isolation of cleavage-stage blastomeres and the study of their developmental potential has been used extensively for analyzing the mechanisms of embryogenesis in vertebrates, including amphibians and echinoderms. We devised a method to isolate 8-cell stage blastomeres in the teleost, shiro-uo, by utilizing its unique cleavage pattern of the horizontal 3rd cleavage plane. Removal of all the upper blastomeres at the 8-cell stage allowed almost normal embryogenesis from the remaining lower blastomeres and yolk cell mass. Isolated upper or lower blastomeres formed vesicles and spherical bodies, which later showed morphological changes during cultivation. Mesoderm formation was detected not only in the cultivated lower blastomeres or whole blastomeres but also in the upper blastomeres isolated from the yolk cell mass at the 8-cell stage, although at a lower frequency than the lower blastomeres. These results indicated the presence of very early signaling for mesoderm induction, which is independent from the currently postulated signals from the yolk syncytial layer at later stages. This also indicated non-equivalence or differentiation of the blastomeres from the very early cleavage stage in teleost embryos.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:14:52