Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Excessive CpG island hypermethylation in cancer cell lines versus primary human malignancies
Autore:
Smiraglia, DJ; Rush, LJ; Fruhwald, MC; Dai, ZY; Held, WA; Costello, JF; Lang, JC; Eng, C; Li, B; Wright, FA; Caligiuri, MA; Plass, C;
Indirizzi:
Ohio State Univ, Dept Mol Virol Immunol & Med Genet, Div Human Canc Genet,Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 n Canc Genet,Columbus, OH 43210 USA Ohio State Univ, Dept Vet Biosci, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 Vet Biosci, Columbus, OH 43210 USA Ohio State Univ, Dept Pathol, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 Dept Pathol, Columbus, OH 43210 USA Ohio State Univ, Dept Otolaryngol, Columbus, OH 43210 USA Ohio State UnivColumbus OH USA 43210 Otolaryngol, Columbus, OH 43210 USA Ohio State Univ, Div Human Genet, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 Human Genet, Columbus, OH 43210 USA Ohio State Univ, Dept Internal Med, Div Hematol & Oncol, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 tol & Oncol, Columbus, OH 43210 USA Univ Munster, Klin & Poliklin Kinderheilkunde Padiat Hamatol On, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 amatol On, D-48149 Munster, Germany Roswell Pk Canc Inst, Dept Mol & Cellular Biol, Buffalo, NY 14263 USA Roswell Pk Canc Inst Buffalo NY USA 14263 lar Biol, Buffalo, NY 14263 USA Univ Calif San Francisco, Dept Neurol Surg, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Brain Tumor Res Ctr, San Francisco, CA 94143 USAUniv Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA
Titolo Testata:
HUMAN MOLECULAR GENETICS
fascicolo: 13, volume: 10, anno: 2001,
pagine: 1413 - 1419
SICI:
0964-6906(20010615)10:13<1413:ECIHIC>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA METHYLATION; HUMAN FIBROBLASTS; PATTERNS; INHIBITION; EXPRESSION; SEQUENCES; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Smiraglia, DJ Ohio State Univ, Dept Mol Virol Immunol & Med Genet, Div Human Canc Genet,420 W 12th Ave,Med Res Facil,Room 470A, Columbus, OH 43210 USA Ohio State Univ 420 W 12th Ave,Med Res Facil,Room 470A Columbus OH USA 43210
Citazione:
D.J. Smiraglia et al., "Excessive CpG island hypermethylation in cancer cell lines versus primary human malignancies", HUM MOL GEN, 10(13), 2001, pp. 1413-1419

Abstract

Cancer cell lines are widely used in many types of cancer research, including studies aimed at understanding DNA hypermethylation of gene promoters in cancer. Hypermethylation of promoters is capable of repressing the expression of tumor suppressor genes and may play a role in the development and/or progression of cancer. Although both primary malignancies and cancer celllines exhibit this epigenetic phenomenon, there has been no direct comparison between them. In order to address this question, we have utilized restriction landmark genomic scanning to measure the hypermethylation phenotypesof cancer cell lines and compared these data with the same analysis performed on primary malignancies. In all cases, cancer cell lines exhibit significantly higher levels of CpG island hypermethylation than the primary malignancies they represent. Colon cancer cell lines are most similar to their respective tumors, with only a ii-fold increase in hypermethylation, while head and neck squamous cell carcinoma cell lines show a 93-fold increase in hypermethylation. Furthermore, >57% of the loci methylated in cell lines are never methylated in 114 primary malignancies studied. Seventy percent of loci hypermethylated in cell lines are hypermethylated in lines from more than one type of cancer. These data indicate that most CPG island hypermethylation observed in cancer cell lines is due to an intrinsic property of cell lines as opposed to the malignant tissue from which they originated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 06:55:08