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Titolo:
Characterization of mesonephric cells that migrate into the XY gonad during testis differentiation
Autore:
Nishino, K; Yamanouchi, K; Naito, K; Tojo, H;
Indirizzi:
Univ Tokyo, Grad Sch Agr & Life Sci, Lab Appl Genet, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 pl Genet, Bunkyo Ku, Tokyo 1138657, Japan
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 2, volume: 267, anno: 2001,
pagine: 225 - 232
SICI:
0014-4827(20010715)267:2<225:COMCTM>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE FETAL TESTIS; WILMS-TUMOR SUPPRESSOR; PRIMORDIAL GERM-CELLS; SEX-DETERMINING GENE; MAMMALIAN GONAD; CORD FORMATION; EXPRESSION; SRY; WT1; EMBRYOS;
Keywords:
cell migration; gonad; Mesonephros; sex determination;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Tojo, H Univ Tokyo, Grad Sch Agr & Life Sci, Lab Appl Genet, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan Univ Tokyo 1-1-1 Yayoi Tokyo Japan 1138657, Tokyo 1138657, Japan
Citazione:
K. Nishino et al., "Characterization of mesonephric cells that migrate into the XY gonad during testis differentiation", EXP CELL RE, 267(2), 2001, pp. 225-232

Abstract

In mouse fetal gonads, sex differentiation begins at 10.5-11.5 days postcoitum (dpc). With XY gonads: of 12.5 dpc, cord-like structures are visible and stromal cells migrate from adjacent mesonephros, unlike in XX gonads. However, the migrated mesonephric cells, except for the endothelial cells, have not been specifically identified because they have not expressed differentiation markers over the course of organ coculture in previous experiments. In this study, we have for the first time succeeded in isolating only themesonephric cells that migrate into the XY gonad from the mesonephros withalive and then cultured these cells in vitro through the use of an organ coculture system using EGFP-transgenic mice and a FAGS Vantage. The migratedand isolated cells were used for morphological and molecular characterization. The migrated mesonephric cells contained three cell forms; a sharp cell form, a round cell form, and a cluster-forming cell. The sharp cells havethe characters of peritubular myoid cells. The round cells and cluster-forming cells have the potential to differentiate into Leydig cells, as some of them are 3 beta -HSD-positive. In in vitro culture of migrated mesonephric cells, the cluster-forming cells proliferated well and then differentiated into round cells, suggesting that the cluster-forming cells may be stem or precursor cells for the round cells. Thus, our findings provide importantinformation related to the migration and differentiation of migrated mesonephric cells in the XY gonad. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 11:53:04