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Titolo:
A gypsy moth (Lymantria dispar, Lepidoptera : Lymantriidae) multinucleocapsid nuclear polyhedrosis virus from France: comparison with a North American and a Korean strain
Autore:
Narang, N; Herard, F; Dougherty, EM; Chen, K; Vega, FE;
Indirizzi:
USDA ARS, Beltsville Agr Res Ctr, Insect Biocontrol Lab, Beltsville, MD 20705 USA USDA ARS Beltsville MD USA 20705 Biocontrol Lab, Beltsville, MD 20705 USA USDA ARS, European Biol Control Lab, F-34988 Montferrier Sur Lez, France USDA ARS Montferrier Sur Lez France F-34988 Montferrier Sur Lez, France
Titolo Testata:
EUROPEAN JOURNAL OF ENTOMOLOGY
fascicolo: 2, volume: 98, anno: 2001,
pagine: 189 - 194
SICI:
1210-5759(2001)98:2<189:AGM(DL>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESTRICTION ENDONUCLEASES; OPTICAL BRIGHTENER; PHYSICAL MAPS; BACULOVIRUS; IDENTIFICATION; ENHANCEMENT; GENOME; GENE; DNA;
Keywords:
biocontrol; microbial control; insect pathogens; baculovirus; NPV; nucleopolyhedrovirus; gypsy moth; Lymantria dispar; fluorescent brightener;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Vega, FE USDA ARS, Beltsville Agr Res Ctr, Insect Biocontrol Lab, Bldg 011A,Rm 214,BARC W, Beltsville, MD 20705 USA USDA ARS Bldg 011A,Rm 214,BARC W Beltsville MD USA 20705 0705 USA
Citazione:
N. Narang et al., "A gypsy moth (Lymantria dispar, Lepidoptera : Lymantriidae) multinucleocapsid nuclear polyhedrosis virus from France: comparison with a North American and a Korean strain", EUR J ENTOM, 98(2), 2001, pp. 189-194

Abstract

As part of a search for natural enemies of the gypsy moth (Lymantria dispar), virus-infected samples were collected near Toulouse, France. Light and electron microscope studies confirmed that the French strain is a multinucleocapsid nuclear polyhedrosis virus (MNPV). In vivo bioassays using the NewJersey strain oft. dispar, and comparing L. dispar MNPV (LdMNPV) strains from France, North America and Korea, showed that the French strain was the least active, whereas the North American strain had the highest activity. The viral efficacy of all strains was enhanced 200 to 1300-fold in the presence of 1% fluorescent brightener. The enhancement was highest in the American strain and lowest in the French strain. French LdMNPV (LdMNPVF) DNA cut with four restriction enzymes (BamHI, EcoRI, HindIII, and NotI) revealed minor fragment size differences, but many similarities when compared to the North American and the Korean strain. PCR amplification of a LdMNPV early gene (G22) was detected in the North American and the Korean strain, but not in the French strain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 22:07:16