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Titolo:
Tissue engineering of small caliber vascular grafts
Autore:
Hoerstrup, SP; Zund, G; Sodian, R; Schnell, AM; Grunenfelder, J; Turina, MI;
Indirizzi:
Univ Zurich Hosp, Cardiovasc Surg Clin, CH-8091 Zurich, Switzerland Univ Zurich Hosp Zurich Switzerland CH-8091 CH-8091 Zurich, Switzerland German Heart Ctr, Berlin, Germany German Heart Ctr Berlin GermanyGerman Heart Ctr, Berlin, Germany Univ Zurich Hosp, Dept Cardiovasc Res, CH-8091 Zurich, Switzerland Univ Zurich Hosp Zurich Switzerland CH-8091 CH-8091 Zurich, Switzerland
Titolo Testata:
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
fascicolo: 1, volume: 20, anno: 2001,
pagine: 164 - 169
SICI:
1010-7940(200107)20:1<164:TEOSCV>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN BLOOD-VESSEL; POLYTETRAFLUOROETHYLENE; CELLS; RECONSTRUCTION; DACRON; VEIN;
Keywords:
tissue engineering; vascular grafts; pulsatile flow;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Hoerstrup, SP Univ Zurich Hosp, Cardiovasc Surg Clin, CH-8091 Zurich, Switzerland Univ Zurich Hosp Zurich Switzerland CH-8091 h, Switzerland
Citazione:
S.P. Hoerstrup et al., "Tissue engineering of small caliber vascular grafts", EUR J CAR-T, 20(1), 2001, pp. 164-169

Abstract

Objective: Previous tissue engineering approaches to create small caliber vascular grafts have been limited by the structural and mechanical immaturity of the constructs. This study uses a novel in vitro pulse duplicator system providing a 'biomimetic' environment during tissue formation to yield more mature, implantable vascular grafts. Methods: Vascular grafts (I.D. 0.5cm) were fabricated from novel bioabsorbable polymers (polyglycolic-acid/poly-4-hydroxybutyrate) and sequentially seeded with ovine vascular myofibroblasts and endothelial cells. After 4 days static culture, the grafts (n = 24) were grown in vitro in a pulse duplicator system (bioreactor) for 4, 7,14, 21, and 28 days. Controls (n = 24) were grown in static culture conditions. Analysis of the neo-tissue included histology, scanning electron microscopy (SEM), and biochemical assays (DNA for cell content, 5-hydroxyproline for collagen). Mechanical testing was performed measuring the burst pressure and the suture retention strength. Results: Histology showed viable, dense tissue in all samples. SEM demonstrated confluent smooth inner surfacesof the grafts exposed to pulsatile flow after 14 days. Biochemical analysis revealed a continuous increase of cell mass and collagen to 21 days compared to significantly lower values in the static controls. The mechanical properties of the pulsed vascular grafts comprised supra-physiological burst strength and suture retention strength appropriate for surgical implantation. Conclusions: This study demonstrates the feasibility of tissue engineering of viable, surgically implantable small caliber vascular grafts and the important effect of a 'biomimetic' in vitro environment on tissue maturation and extracellular matrix formation. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:36:00