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Titolo:
Impact of copper on biomonitoring enzyme ethoxyresorufin-o-deethylase in cultured catfish hepatocytes
Autore:
Ghosh, MC; Ghosh, R; Ray, AK;
Indirizzi:
Bose Inst, Dept Anim Physiol, Calcutta 7009054, W Bengal, India Bose InstCalcutta W Bengal India 7009054 lcutta 7009054, W Bengal, India
Titolo Testata:
ENVIRONMENTAL RESEARCH
fascicolo: 2, volume: 86, anno: 2001,
pagine: 167 - 173
SICI:
0013-9351(200106)86:2<167:IOCOBE>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED CYTOCHROME-P450 METABOLISM; RAT HEPATOCYTES; RAINBOW-TROUT; HEAVY-METALS; AH RECEPTOR; FISH; METALLOTHIONEIN; GLUTATHIONE; INDUCTION; ZINC;
Keywords:
carbofuran; beta-napthoflavone; copper; glutathione; EROD; fish;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Ghosh, MC Bose Inst, Dept Anim Physiol, P-1-12 CIT Scheme VII M, Calcutta 7009054, WBengal, India Bose Inst P-1-12 CIT Scheme VII M Calcutta W BengalIndia 7009054
Citazione:
M.C. Ghosh et al., "Impact of copper on biomonitoring enzyme ethoxyresorufin-o-deethylase in cultured catfish hepatocytes", ENVIR RES, 86(2), 2001, pp. 167-173

Abstract

The enzyme ethoxyresorufin-o-deethyl ase (EROD) of the cytochrome P4501A family (CYP1A) in fish liver is increasingly being used as a molecular marker for qualitative and quantitative estimation of aquatic pollution throughout the world. The regulation and expression of this enzyme protein is very important from the toxicological point of view. The regulation of gene expression for this enzyme is mediated by the aromatic hydrocarbon receptor. Inaddition, cellular glutathione status influences expression of CYP1A. In this study, we explored the relationships among glutathione, EROD, and copper in cultured hepatocytes from Indian catfish. EROD activity in cultured hepatocytes was induced by carbofuran ICF), a widely used agricultural pesticide, and by beta -napthoflavone (BNF), a known inducer of CYP1A. Addition of copper into the culture media of hepatocytes inhibited EROD activity significantly. The activity of EROD elevated by CF and BNF was inhibited in hepatocytes pretreated with CF and BNF exposed to CuSO4. This effect was reflected in the glutathione status of the cells. The level of glutathione was increased by 3.4 and 3.0 times in hepatocytes treated with CF and BNF, respectively. These levels were inhibited in hepatocytes exposed to CuSO4. Thus,copper interactions with glutathione may play a role in regulating EROD inhepatocytes. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 15:50:17