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Titolo:
Evidence for a role of protein kinase C in FGF signal transduction in the developing chick limb bud
Autore:
Lu, HC; Swindell, EC; Sierralta, WD; Eichele, G; Thaller, C;
Indirizzi:
Mac Planck Inst Expt Endocrinol, D-30625 Hannover, Germany Mac Planck InstExpt Endocrinol Hannover Germany D-30625 nnover, Germany Baylor Coll Med, Dev Biol Program, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 Biol Program, Houston, TX 77030 USA Baylor Coll Med, Div Neurosci, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 Div Neurosci, Houston, TX 77030 USA Baylor Coll Med, V & M McLean Dept Biochem, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 Dept Biochem, Houston, TX 77030 USA
Titolo Testata:
DEVELOPMENT
fascicolo: 13, volume: 128, anno: 2001,
pagine: 2451 - 2460
SICI:
0950-1991(200107)128:13<2451:EFAROP>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; MOTOR-NEURON INDUCTION; RETINOIC ACID; VERTEBRATE LIMB; SONIC-HEDGEHOG; INTRACELLULAR RECEPTOR; POLARIZING ACTIVITY; ESTRADIOL-RECEPTOR; ADDITIONAL LIMB; HOMEOBOX GENES;
Keywords:
FGF; FGFR; protein kinase C; RACK1; retinoids; sonic hedgehog; chick;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Eichele, G Mac Planck Inst Expt Endocrinol, Feodor Lynen Str 7, D-30625 Hannover, Germany Mac Planck Inst Expt Endocrinol Feodor Lynen Str 7 Hannover Germany D-30625
Citazione:
H.C. Lu et al., "Evidence for a role of protein kinase C in FGF signal transduction in the developing chick limb bud", DEVELOPMENT, 128(13), 2001, pp. 2451-2460

Abstract

In developing limbs, numerous signaling molecules have been identified butless is know about the mechanisms by which such signals direct patterning. We have explored signal transduction pathways in the chicken limb bud, A cDNA encoding RACK1, a protein that binds and stabilizes activated protein kinase C (PKC), was isolated in a screen for genes induced by retinoic acid (RA) in the chick wing bud. Fibroblast growth factor (FGF) also induced RACK1 and such induction of RACK1 expression was accompanied by a significant augmentation in the number of active PKC molecules and an elevation of PKC enzymatic activity. This suggests that PKCs mediate signal transduction in the limb bud. Application of chelerythrine, a potent PKC inhibitor, to the presumptive wing region resulted in buds that did not express sonic hedgehog (Shh) and developed into wings that were severely truncated. This observation suggests that the expression of Shh depends on PKCs, Providing ectopicSHH protein, RA or ZPA grafts overcome the effects of blocking PKC with chelerythrine and resulted in a rescue of the wing morphology, Taken together, these findings suggest that the responsiveness of Shh to FGF is mediated,at least in part, by PKCs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 01:57:15