Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of iloprost, a stable prostacyclin analog, on intestinal leukocyteadherence and microvascular blood flow in rat experimental endotoxemia
Autore:
Lehmann, C; Konig, JP; Dettmann, J; Birnbaum, J; Kox, WJ;
Indirizzi:
Humboldt Univ, Klinikum Charite, Klin Anasthesiol & Operat Intens Med, Berlin, Germany Humboldt Univ Berlin Germany esiol & Operat Intens Med, Berlin, Germany
Titolo Testata:
CRITICAL CARE MEDICINE
fascicolo: 7, volume: 29, anno: 2001,
pagine: 1412 - 1416
SICI:
0090-3493(200107)29:7<1412:EOIASP>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLE-ORGAN-FAILURE; GASTROINTESTINAL-TRACT; SEPSIS; PROSTAGLANDINS; EXPRESSION; MUSCLE; SHOCK; MICROCIRCULATION; MACROPHAGES; INHIBITION;
Keywords:
animal experiments; rats; endotoxemia; lipopolysaccharide; iloprost; prostacyclin; intravital fluorescence microscopy; intestine; leukocyte adhesion; laser Doppler flowmetry; cytokines;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Lehmann, C Humboldt Univ, Klinikum Charite, Klin Anasthesiol & Operat Intens Med, Campus Charite Mitte, Berlin, Germany Humboldt Univ Campus Charite Mitte Berlin Germany in, Germany
Citazione:
C. Lehmann et al., "Effects of iloprost, a stable prostacyclin analog, on intestinal leukocyteadherence and microvascular blood flow in rat experimental endotoxemia", CRIT CARE M, 29(7), 2001, pp. 1412-1416

Abstract

Objective: To investigate the effects of iloprost, a stable prostacyclin analog, on leukocyte adherence in intestinal venules and intestinal microvascular blood flow in experimental endotoxemia. Design: Prospective, randomized, controlled animal study. Setting: Experimental laboratory. Subjects: Twenty-one male Wistar rats weighing 190 +/- 40 g. Interventions: The rats were divided equally into three groups: the first was a control group; the second received endotoxin (20 mg/kg lipopolysaccharide from Escherichia cell O55:B5 intravenously); and the third received endotoxin and intravenous iloprost infusion (2 ng.kg(-1).min(-1)). Measurements and Main Results: The distal small intestine of the animals was examined by using intravital fluorescence videomicroscopy 2 hrs after endotoxin challenge. Leukocytes were stained in vivo by means of rhodamine 6G. Intestinal microvascular blood flow was measured by laser Doppler flowmetry in the terminal ileum. Iloprost treatment significantly attenuated the count of adherent leukocytes in collecting venules (control, 61 +/- 10 n/mm(2); lipopolysaccharide, 364 +/- 60 n/mm(2); iloprast, 232 +/- 29 n/mm(2); p< .05) and in postcapillary venules (control, 96 +/- 14 n/mm(2); lipopolysaccharide, 470 +/- 21 n/mm(2); iloprost 390 +/- 41 n/mm(2); p < .05). Intestinal microvascular blood flow was decreased significantly in the lipopolysaccharide group (-49%), whereas iloprost-treated animals showed no significant difference compared with the control group. Conclusions: The study demonstrated that administration of iloprost attenuated leukocyte adherence in postcapillary and collecting intestinal venulesand improved intestinal microvascular blood flow. Thus, iloprost treatmentmay impact endotoxin-induced intestinal injury.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:20:25