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Titolo:
Leukocyte attack in a 3D human coronary in-vitro model
Autore:
Voisard, R; Voglic, S; Baur, R; Susa, M; Koenig, W; Hombach, V;
Indirizzi:
Univ Ulm, Dept Internal Med Cardiol 2, D-89081 Ulm, Germany Univ Ulm UlmGermany D-89081 nternal Med Cardiol 2, D-89081 Ulm, Germany Univ Ulm, Dept Med Microbiol, D-89081 Ulm, Germany Univ Ulm Ulm Germany D-89081 m, Dept Med Microbiol, D-89081 Ulm, Germany
Titolo Testata:
CORONARY ARTERY DISEASE
fascicolo: 5, volume: 12, anno: 2001,
pagine: 401 - 411
SICI:
0954-6928(200108)12:5<401:LAIA3H>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; TUMOR-NECROSIS-FACTOR; INTERCELLULAR-ADHESION MOLECULE-1; HUMAN VASCULAR CELLS; ATHEROSCLEROTIC PLAQUES; ENDOTHELIAL-CELLS; T-LYMPHOCYTES; FACTOR-ALPHA; EXPRESSION; PROLIFERATION;
Keywords:
restenosis; atherosclerosis; leukocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Voisard, R Univ Ulm, Dept Internal Med Cardiol 2, Robert Koch Str 8, D-89081 Ulm, Germany Univ Ulm Robert Koch Str 8 Ulm Germany D-89081 81 Ulm, Germany
Citazione:
R. Voisard et al., "Leukocyte attack in a 3D human coronary in-vitro model", CORON ART D, 12(5), 2001, pp. 401-411

Abstract

Objective The importance of peripheral blood leukocytes for the development of early atherosclerosis and restenosis has confronted cardiologists withclassical hematologic issues. Three-dimensional human coronary in-vitro units of leukocyte attack (3DLA-units) open the field for exact studies of leukocyte attack and its subsequent effects on human medial coronary smooth muscle cells (HCMSMC). Methods Central part of 3DLA-units are polycarbonate membranes with a poresize of 5 mum that correspond to the internal elastic membrane. Human coronary endothelial cells (HCAEC) were cultured on one side of the membranes, HCMSMC on the other side. Before leukocyte attack expression of adhesion molecules was up-regulated by tumour necrosis factor-alpha (TNF-alpha). Leukocyte attack was mimicked by selective adding of human monocytes (MC), respectively human CD4(+)-lymphocytes (CD4(+)-LC) to the HCAEC side of the 3DLA-units. Three-dimensional leukocyte attack units were fixed and stained after a period of 30 min, 1, 2, 3, 4, 6, and 24 h. Cell divisions of HCMSMC were analysed by measuring the uptake of bromodeoxyuridine (BrdU). Results Monocytes were able to adhere to the endothelial surface, pass through the filter-pores, and penetrate the HCMSMC side of the 3DLA-units. Human CD4(+)-lymphocytes (CD4(+)-LC) only attached to the HCAEC side, and no chemotaxis to the HCMSMC side was detected. Proliferation of HCMSMC was increased 2.9-fold (P < 0.001) after selective MC-attack and 3.5-fold after selective MC-attack and TNF-<alpha> stimulus. No significant increase was found after selective CD4(+)-LC attack, a significant increase (2.1-fold; P< 0.001) was seen after selective CD4(+)-LC attack and TNF-<alpha> stimulus. Conclusions Within the given limitations of the model the study emphasizesa predominance of MC in comparison to CD4(+)-LC in the process of adhesion, chemotaxis, and triggered reactive proliferation of co-cultured HCMSMC within the first 24 h after leukocyte attack. 3DLA-units offer an elegant method to study directly the effects of intravascular and intramural treatmentstrategies. Coron Artery Dis 12:401-411 (C) 2001 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 04:09:04