Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Regulatory role of IL-1 beta in the expression of IL-6 and IL-8 in human corneal epithelial cells during Pseudomonas aeruginosa colonization
Autore:
Xue, ML; Willcox, MDP; Lloyd, A; Wakefield, D; Thakur, A;
Indirizzi:
Univ New S Wales, Cooperat Res Ctr Eye Res & Technol, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 l, Sydney, NSW 2052, Australia Univ New S Wales, Sch Pathol, Inflammat Res Unit, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 t, Sydney, NSW 2052, Australia
Titolo Testata:
CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
fascicolo: 3, volume: 29, anno: 2001,
pagine: 171 - 174
SICI:
1442-6404(200106)29:3<171:RROIBI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERLEUKIN-1 RECEPTOR ANTAGONIST; MACROPHAGE-INFLAMMATORY PROTEIN-2; INFECTION;
Keywords:
cytokines; human corneal epithelial cell; IL-1 beta; Pseudomonas aeruginosa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Xue, ML Univ New S Wales, Cooperat Res Ctr Eye Res & Technol, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 y, NSW 2052, Australia
Citazione:
M.L. Xue et al., "Regulatory role of IL-1 beta in the expression of IL-6 and IL-8 in human corneal epithelial cells during Pseudomonas aeruginosa colonization", CLIN EXP OP, 29(3), 2001, pp. 171-174

Abstract

Pseudomonas aeruginosa is a virulent pathogen and is frequently associatedwith bacterial keratitis. Recent studies have shown that high levels of interleukin (IL)-1 beta and macrophage inflammatory protein-2 are associated with the severity of corneal infection. Interleukin-1 beta is a principal inflammatory mediator. Understanding the regulatory role of IL-1 beta would provide better understanding of host responses during P. aeruginosa cornealinfection. A human corneal epithelial (HCE) cell line and three P. aeruginosa strains were used in this experiment. Confluent HCE cells were challenged with P. aeruginosa and monoclonal antihuman IL-1 beta antibody (IL-1 beta mAb). The culture supernatants were collected for measuring cytotoxicity and protein levels of IL-1 beta, IL-8 and IL-6 by enzyme-linked immunosorbent assay. Results showed that HCE cells expressed low levels of IL-1 beta and high levels of IL-6 and IL-8 during P. aeruginosa colonization. Paer1-colonized HCE cells produced higher levels of IL-1 beta, IL-6 and IL-8 protein compared to those produced by 6206- and 6294- colonized HCE cells. Administration of IL-1 beta mAb decreased the production of IL-8 and IL-6. In conclusion, P. aeruginosa-colonized HCE cells produced low levels of IL-1 betaand high levels of IL-6 and IL-8. Neutralizing IL-1 beta protein significantly downregulated the production of IL-8 and IL-6.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:29:58