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Titolo:
Aldosterone induces angiotensin-converting-enzyme gene expression in cultured neonatal rat cardiocytes
Autore:
Harada, E; Yoshimura, M; Yasue, H; Nakagawa, O; Nakagawa, M; Harada, M; Mizuno, Y; Nakayama, M; Shimasaki, Y; Ito, T; Nakamura, S; Kuwahara, K; Saito, Y; Nakao, K; Ogawa, H;
Indirizzi:
Kumamoto Univ, Sch Med, Dept Cardiovasc Med, Kumamoto 8608556, Japan Kumamoto Univ Kumamoto Japan 8608556 iovasc Med, Kumamoto 8608556, Japan Kumamoto Aging Res Inst, Div Cardiol, Kumamoto, Japan Kumamoto Aging Res Inst Kumamoto Japan st, Div Cardiol, Kumamoto, Japan Kyoto Univ, Grad Sch Med, Dept Med & Clin Sci, Kyoto, Japan Kyoto Univ Kyoto Japan Grad Sch Med, Dept Med & Clin Sci, Kyoto, Japan
Titolo Testata:
CIRCULATION
fascicolo: 2, volume: 104, anno: 2001,
pagine: 137 - 139
SICI:
0009-7322(20010710)104:2<137:AIAGEI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEART-FAILURE; HYPERTROPHY; TISSUE; MINERALOCORTICOIDS; HYPERTENSION; FIBROSIS; SYSTEM;
Keywords:
angiotensin; aldosterone; polymerase chain reaction; cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Yoshimura, M Kumamoto Univ, Sch Med, Dept Cardiovasc Med, 1-1-1 Honjo, Kumamoto 8608556, Japan Kumamoto Univ 1-1-1 Honjo Kumamoto Japan 8608556 8556, Japan
Citazione:
E. Harada et al., "Aldosterone induces angiotensin-converting-enzyme gene expression in cultured neonatal rat cardiocytes", CIRCULATION, 104(2), 2001, pp. 137-139

Abstract

Background-The cardiac renin-angiotensin-aldosterone system is activated in failing hearts in proportion to the severity of the disease. We hypothesized that a positive feedback mechanism might exist within this system and contribute to the progression of the heart failure. Methods and Results-To test this hypothesis, we examined whether angiotensin Il or aldosterone induces the expression of angiotensin-converting-enzyme (ACE) mRNA in cultured neonatal rat ventricular cardiocytes. Expression of ACE mRNA was detected and quantified using real-time reverse transcription-polymerase chain reaction. Exposure to angiotensin II (10(-5) mol/L) for 24 hours had no significant effect on the expression of ACE mRNA (0.7 +/-0.5-fold versus control, P=NS), but similar treatment with aldosterone (10(-5) mol/L) induced a 23.3 +/- 7.9-fold increase (P <0.01) in ACE mRNA expression. The effect of aldosterone was both time- (maximal effect, 24 hours) and dose-dependent (EC50, 4X10(-7) mol/L), and it was significantly (P<0.01) inhibited by spironolactone, a specific mineralocorticoid receptor antagonist. Conclusions-Aldosterone upregulates ACE mRNA expression, which is blocked by spironolactone in neonatal rat cardiocytes. Thus, spironolactone may suppress the progression of heart failure by blocking the effects of aldosterone and angiotensin II.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:39:17