Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Mitochondrial targeting of JNK/SAPK in the phorbol ester response of myeloid leukemia cells
Autore:
Ito, Y; Mishra, NC; Yoshida, K; Kharbanda, S; Saxena, S; Kufe, D;
Indirizzi:
Harvard Univ, Dana Farber Canc Inst, Sch Med, Boston, MA 02115 USA HarvardUniv Boston MA USA 02115 Canc Inst, Sch Med, Boston, MA 02115 USA Lovelace Resp Res Inst, Albuquerque, NM 87115 USA Lovelace Resp Res Inst Albuquerque NM USA 87115 Albuquerque, NM 87115 USA
Titolo Testata:
CELL DEATH AND DIFFERENTIATION
fascicolo: 8, volume: 8, anno: 2001,
pagine: 794 - 800
SICI:
1350-9047(200108)8:8<794:MTOJIT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; INTERNUCLEOSOMAL DNA FRAGMENTATION; SIGNAL-TRANSDUCTION PATHWAY; EARLY GROWTH RESPONSE-1; MONOCYTIC DIFFERENTIATION; CYTOCHROME-C; MACROPHAGE DIFFERENTIATION; GENE-EXPRESSION; JUN EXPRESSION; BCL-X(L);
Keywords:
terminal differentiation; TPA; protein kinase C beta; stress-activated protein kinase; mitochondria; Bcl-x(L);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Kufe, D Harvard Univ, Dana Farber Canc Inst, Sch Med, Boston, MA 02115 USAHarvard Univ Boston MA USA 02115 t, Sch Med, Boston, MA 02115 USA
Citazione:
Y. Ito et al., "Mitochondrial targeting of JNK/SAPK in the phorbol ester response of myeloid leukemia cells", CELL DEAT D, 8(8), 2001, pp. 794-800

Abstract

Treatment of human U-937 myeloid leukemia cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) is associated with activation of the stress-activatedprotein kinase (SAPK) and induction of terminal monocytic differentiation,The present studies demonstrate that TPA targets SAPK to mitochondria by amechanism dependent on activation of protein kinase C (PKC) beta. Translocation of SAPK to mitochondria in response to TPA is associated with releaseof cytochrome c, caspase-3 activation and induction of apoptosis, The results show that IPA induces the association of SAPK with the mitochondrial anti-apoptotic Bcl-x(L) protein, Overexpression of Bcl-xL attenuated the apoptotic response to TPA treatment. Moreover, expression of Bcl-xL mutated at sites of SAPK phosphorylation (Thr-47, -115) was more effective than wildtype Bcl-xL in abrogating TPA-induced cytochrome c release and apoptosis, By contrast, expression of Bcl-xL had little effect on induction of the monocytic phenotype, These findings indicate that myeloid leukemia cells respond to TPA with targeting of SAPK to mitochondria and that this response contributes to terminal differentiation through the release of cytochrome c and induction of apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/10/20 alle ore 01:33:39