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Titolo:
Gracilis muscle flap with a tissue-engineered lining For experimental bladder wall reconstruction
Autore:
Schoeller, T; Lille, S; Bauer, T; Piza-Katzer, H; Wechselberger, G;
Indirizzi:
Leopold Franzens Univ, Univ Hosp Innsbruck, Ludwig Boltzmann Inst Qual Control Plast Surg, Dept Plast & Reconstruct Surg, Innsbruck, Austria Leopold Franzens Univ Innsbruck Austria struct Surg, Innsbruck, Austria So Illinois Univ, Sch Med, Inst Plast & Reconstruct Surg, Springfield, IL USA So Illinois Univ Springfield IL USA econstruct Surg, Springfield, IL USA
Titolo Testata:
BJU INTERNATIONAL
fascicolo: 1, volume: 88, anno: 2001,
pagine: 104 - 109
SICI:
1464-4096(200107)88:1<104:GMFWAT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL TRANSPLANTATION; UROTHELIAL CELLS; AUGMENTATION; SUBSTITUTE; REGENERATION; EXPRESSION; SEGMENTS;
Keywords:
gracilis muscle; muscle flaps; tissue engineering; urothelial culture; bladder wall reconstruction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Schoeller, T Innsbruck Univ, Klin Plast & Wiederherstellungschirurg, Anichstr 35, A-6020 Innsbruck, Austria Innsbruck Univ Anichstr 35 Innsbruck Austria A-6020 Austria
Citazione:
T. Schoeller et al., "Gracilis muscle flap with a tissue-engineered lining For experimental bladder wall reconstruction", BJU INT, 88(1), 2001, pp. 104-109

Abstract

Objective To assess a pedicled gracilis muscle flap prelaminated with autologous, in vitro-expanded urothelial cells to reconstruct an entire supratrigonal bladder-wall defect in rats. Materials and methods A gracilis muscle flap was harvested from 36 male Wistar rats, transposed into the abdomen and wrapped around a silicon-block space holder, Urothelial cells were harvested and expanded ex vivo. Cells were then suspended in fibrin glue and seeded into the gracilis muscle pocket. One week later this pre-laminated nap was transposed into a surgically created supratrigonal bladder-wall defect. All animals underwent such a pre-laminated gracilis nap bladder reconstruction and were categorized into three experimental groups. All surviving animals with urothelial-culture pre-laminated gracilis flap bladder reconstruction were killed 12 weeks (group I)later. Control rats had gracilis naps with no cell seeding and treated only with fibrin glue (group 2) or a standard culture medium (group 3) before reconstruction. Results Specimens stained with haematoxylin and eosin, and a specific immunohistochemical staining (AE(1) and AE(3)-anti-cytokeratin monoclonal antibody stain) showed a continuous, multilayered functioning urothelial lining along the transposed pre-laminated gracilis flap in group 1. All animals ingroup 1 with an intact urothelial lining on the gracilis muscle survived, in contrast to most animals in groups 2 and 3, where eight and all 12 animals died, respectively. The surviving four animals in group 2 had no detectable urothelial lining. Conclusion Successful urinary reconstruction requires a contractile neo-reservoir resistant to resorption over time and a stable, protective urothelial lining. A gracilis muscle flap can be seeded with autologous cultured urothelial cells suspended in fibrin glue. This pre-laminated flap can be safely transposed on its pedicle and be successfully integrated into the remaining bladder wall, with a urothelial lining and the potential to contract. Further studies in larger animals, with a urodynamic assessment, are warranted to determine if this type of bladder-wall replacement technique is suitable for urinary reconstruction in humans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 13:53:10