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Titolo:
Controlling lipase enantioselectivity for organic synthesis
Autore:
Berglund, P;
Indirizzi:
Royal Inst Technol, KTH, Dept Biotechnol, SE-10044 Stockholm, Sweden RoyalInst Technol Stockholm Sweden SE-10044 SE-10044 Stockholm, Sweden
Titolo Testata:
BIOMOLECULAR ENGINEERING
fascicolo: 1, volume: 18, anno: 2001,
pagine: 13 - 22
SICI:
1389-0344(200108)18:1<13:CLEFOS>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANDIDA-RUGOSA LIPASE; DYNAMIC KINETIC RESOLUTION; CATALYZED TRANSESTERIFICATION REACTIONS; CONTROLLED WATER ACTIVITY; X-RAY CRYSTALLOGRAPHY; CHIRAL ACYL DONOR; DIRECTED EVOLUTION; ENANTIOMERIC RATIO; 2-PHENOXYPROPIONIC ACIDS; 2-METHYLALKANOIC ACIDS;
Keywords:
lipase; biocatalysis; enantio-reversal; enantioselectivity enhancement; medium engineering; substrate engineering; directed evolution; DNA shuffling;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
136
Recensione:
Indirizzi per estratti:
Indirizzo: Berglund, P Royal Inst Technol, KTH, Dept Biotechnol, SE-10044 Stockholm, Sweden Royal Inst Technol Stockholm Sweden SE-10044 ockholm, Sweden
Citazione:
P. Berglund, "Controlling lipase enantioselectivity for organic synthesis", BIOMOL ENG, 18(1), 2001, pp. 13-22

Abstract

Lipases are used frequently as chiral catalysts in the synthesis of various fine chemicals and intermediates. The increasing need of compounds with high stereochemical purity requires catalysts with an improved and controlled performance. This overview emphasizes some important aspects for the control of lipase enantioselectivity and some examples where the enantioselectivity has been altered or reversed are highlighted. However, in several of these cases the complete explanation for the altered or reversed enantioselectivity remains unclear and needs to be solved. Three different strategies (engineering of the reaction medium, the substrate molecule, and the enzyme) for exploring lipase enantioselectivity at a molecular level are discussed and summarized. These three different approaches represent powerful toolsfor understanding the molecular basis for lipase enantioselective catalysis and can guide the rational improvement and tailoring of catalyst performance. By combining approaches from chemistry and biology much is learnt about the most important parameters controlling lipase enantioselectivity for organic synthesis. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:16:28