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Titolo:
Comparison of two cysteine endopeptidases from Pseudananas macrodontes (Morr.) Harms (Bromeliaceae)
Autore:
Lopez, LMI; Sequeiros, C; Trejo, SA; Pardo, MF; Caffini, NO; Natalucci, CL;
Indirizzi:
Natl Univ La Plata, Fac Ciencias Exactas, Dept Ciencias Biol, LIPROVE, RA-1900 La Plata, Argentina Natl Univ La Plata La Plata Argentina RA-1900 A-1900 La Plata, Argentina
Titolo Testata:
BIOLOGICAL CHEMISTRY
fascicolo: 5, volume: 382, anno: 2001,
pagine: 875 - 878
SICI:
1431-6730(200105)382:5<875:COTCEF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-SEQUENCE; CARICA-CANDAMARCENSIS HOOK; STEM BROMELAIN; UNRIPE FRUITS; PROTEINASES; PURIFICATION; EXPRESSION; PROTEASES; ANANAIN; LATEX;
Keywords:
enzyme purification and characterization; N-terminal sequence homology; plant endopeptidases; thiol proteases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Caffini, NO Natl Univ La Plata, Fac Ciencias Exactas, Dept Ciencias Biol, LIPROVE, CC 711, RA-1900 La Plata, Argentina Natl Univ La Plata CC 711 La Plata Argentina RA-1900 rgentina
Citazione:
L.M.I. Lopez et al., "Comparison of two cysteine endopeptidases from Pseudananas macrodontes (Morr.) Harms (Bromeliaceae)", BIOL CHEM, 382(5), 2001, pp. 875-878

Abstract

The properties of two cysteine peptidases (macro-dontain I and II) isolated from fruits of Pseudananas macrodontes have been compared. The enzymes showed optimum pH ranges near neutrality and were inhibited by E-64 and othercysteine peptidase inhibitors. Molecular masses were 23459 and 23703 kDa, the isoelectric points were 6.1 and 5.9, and the K-m values were 13.4 and 8.9 muM (Bz-Phe-Val-Arg-AMC) for macrodontain I and II, respectively. N-alpha CBZ-L-amino acid p-nitrophenyl esters were tested for both enzymes. The N-terminal sequences of both proteases differed slightly and showed high sequence similarity to other pineapple stem-derived cysteine endopeptidases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 12:44:32