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Titolo:
Role of calpain in human sperm activated by progesterone for fertilization
Autore:
Ozaki, Y; Blomgren, K; Ogasawara, MS; Aoki, K; Furuno, T; Nakanishi, M; Sasaki, M; Suzumori, K;
Indirizzi:
Nagoya City Univ, Sch Med, Dept Obstet & Gynecol, Nagoya, Aichi 4678601, Japan Nagoya City Univ Nagoya Aichi Japan 4678601 Nagoya, Aichi 4678601, Japan Univ Gothenburg, Perinatal Ctr, Inst Physiol & Pharmacol, S-40530 Gothenburg, Sweden Univ Gothenburg Gothenburg Sweden S-40530 ol, S-40530 Gothenburg, Sweden Nagoya City Univ, Dept Biochem, Nagoya, Aichi 4678601, Japan Nagoya City Univ Nagoya Aichi Japan 4678601 Nagoya, Aichi 4678601, Japan Jhosai Municiap Hosp Nagoya City, Dept Obstet & Gynecol 2, Nagoya, Aichi 4530815, Japan Jhosai Municiap Hosp Nagoya City Nagoya Aichi Japan 4530815 530815, Japan Nagoya City Univ, Fac Pharmaceut Sci, Nagoya, Aichi 4678603, Japan Nagoya City Univ Nagoya Aichi Japan 4678603 Nagoya, Aichi 4678603, Japan
Titolo Testata:
BIOLOGICAL CHEMISTRY
fascicolo: 5, volume: 382, anno: 2001,
pagine: 831 - 838
SICI:
1431-6730(200105)382:5<831:ROCIHS>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACROSOME REACTION; NEUTRAL PROTEASE; MESSENGER-RNA; INTRACELLULAR CALCIUM; CALPASTATIN ISOFORMS; HAMSTER SPERMATOZOA; CELL-MEMBRANE; PROTEOLYSIS; RECEPTOR; ISCHEMIA;
Keywords:
calcium-dependent cysteine protease; capacitation; human sperm; intracellular calcium concentration; progesterone; protease inhibitor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Ozaki, Y Nagoya City Univ, Sch Med, Dept Obstet & Gynecol, Nagoya, Aichi 4678601, Japan Nagoya City Univ Nagoya Aichi Japan 4678601 Aichi 4678601, Japan
Citazione:
Y. Ozaki et al., "Role of calpain in human sperm activated by progesterone for fertilization", BIOL CHEM, 382(5), 2001, pp. 831-838

Abstract

We evaluated the activation of mu -calpain in progesterone-activated humansperm. Semen collected from fertile donors with informed consent was liquefied and subjected to percoll gradient centrifugation. After exposure to different concentrations of progesterone, the samples were used for immunostaining, SDS-PAGE and Western blot analysis. An increase of the intracellularfree calcium concentration in the sperm following the addition of progesterone was observed using fura-e AM. Immunostaining using an antibody againstactive mu -calpain produced 6 distinct staining patterns: (1) the acrosome, (2) an equatorial segment, (3) the whole head, (4) the neck, (5) the neckand tail or (6) unstained sperm. After addition of progesterone, the predominant type changed from the neck type (90%) to the neck and tail type (79%). Western blot analysis using a pro-mu -calpain and a mu -calpain domain III antibody revealed autodigestion of mu -calpain, indicating activation byprogesterone. Using calpain-specific inhibitors it was shown that calpain activation contributes to sperm motility as well as to the acrosome reaction. These results suggest the possibility that activation of mu -calpain in human sperm by progesterone plays an important role in fertilization.

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Documento generato il 18/01/20 alle ore 13:14:53