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Titolo:
Cathepsin B/cystatin C complex levels in sera from patients with lung and colorectal cancer
Autore:
Zore, I; Krasovec, M; Cimerman, N; Kuhelj, R; Werle, B; Nielsen, HJ; Brunner, N; Kos, J;
Indirizzi:
Krka DD, R&D Div, Dept Biochem Res & Drug Design, Ljubljana 1000, SloveniaKrka DD Ljubljana Slovenia 1000 & Drug Design, Ljubljana 1000, Slovenia Josef Stefan Inst, Dept Biochem & Mol Biol, Ljubljana 1000, Slovenia JosefStefan Inst Ljubljana Slovenia 1000 Biol, Ljubljana 1000, Slovenia Thoraxhosp Heidelberg GMBh, Dept Clin Chem & Bacteriol, D-69123 Heidelberg, Germany Thoraxhosp Heidelberg GMBh Heidelberg Germany D-69123 eidelberg, Germany Rigshosp, Finsen Lab, DK-2100 Copenhagen, Denmark Rigshosp Copenhagen Denmark DK-2100 sen Lab, DK-2100 Copenhagen, Denmark Hvidovre Univ Hosp, Dept Surg Gastroenterol, DK-2650 Hvidovre, Denmark Hvidovre Univ Hosp Hvidovre Denmark DK-2650 l, DK-2650 Hvidovre, Denmark
Titolo Testata:
BIOLOGICAL CHEMISTRY
fascicolo: 5, volume: 382, anno: 2001,
pagine: 805 - 810
SICI:
1431-6730(200105)382:5<805:CBCCLI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYSTEINE PROTEINASE-INHIBITORS; LINKED-IMMUNOSORBENT-ASSAY; B-LIKE ACTIVITY; UROKINASE-TYPE; CYSTATIN-C; PLASMINOGEN-ACTIVATOR; PEPTIDASE INHIBITORS; TUMOR PROGRESSION; PROGNOSIS; DIAGNOSIS;
Keywords:
cathepsin B; colorectal cancer; complex; cystatin C; lung cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Kos, J Krka DD, R&D Div, Dept Biochem Res & Drug Design, Ljubljana 1000, Slovenia Krka DD Ljubljana Slovenia 1000 Design, Ljubljana 1000, Slovenia
Citazione:
I. Zore et al., "Cathepsin B/cystatin C complex levels in sera from patients with lung and colorectal cancer", BIOL CHEM, 382(5), 2001, pp. 805-810

Abstract

A sandwich-type ELISA has been developed for quantification of the complexbetween the cysteine proteinase cathepsin B (CB) and its reversible tight-binding inhibitor cystatin C (CC) in normal and pathological sera. The assay is based on a combination of catching Ab (3E1), raised against CB, and a horseradish peroxidase-labelled detection Ab (1A2), raised against CC. Onlythe CB/CC complex is able to evoke a signal in this assay. The detection limit of the assay was 15.5 nM and the working range between 31.3-200 nM. The within and between-run coefficients of variance (CV) varied from 4.7% to 9.4% and 11% to 12.8%, respectively, demonstrating satisfactory reproducibility of the method. The concentration of the CB/CC complex was determined in sera from 90 healthy controls, 32 patients with non-cancerous lung diseases, 148 patients with lung and 32 patients with colorectal cancer. The CB/CC complex was significantly less abundant in sera of patients bearing malignant lung tumours than in those with non-cancerous lung diseases or healthycontrols (p <0.001). In colorectal cancer sera its level was significantlylower in advanced stages C and D than in early Dukes' stages A and B (p=0.02). Our results show that the increased levels of CB in malignant sera arenot impaired effectively by CC and support the hypothesis of hindered inhibitory capability during cancer progression.

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Documento generato il 06/04/20 alle ore 08:15:21