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Titolo:
Immunochemical localisation of cathepsin S, cathepsin L and MHC class II-associated p41 isoform of invariant chain in human lymph node tissue
Autore:
Zavasnik-Bergant, V; Sekirnik, A; Golouh, R; Turk, V; Kos, J;
Indirizzi:
Jozef Stefan Inst, Dept Biochem & Mol Biol, Ljubljana 1000, Slovenia JozefStefan Inst Ljubljana Slovenia 1000 Biol, Ljubljana 1000, Slovenia Inst Oncol, Dept Pathol, Ljubljana 1000, Slovenia Inst Oncol Ljubljana Slovenia 1000 Dept Pathol, Ljubljana 1000, Slovenia Dept Biochem Res & Drug Design, R&D Div, Ljubljana 1000, Slovenia Dept Biochem Res & Drug Design Ljubljana Slovenia 1000 na 1000, Slovenia
Titolo Testata:
BIOLOGICAL CHEMISTRY
fascicolo: 5, volume: 382, anno: 2001,
pagine: 799 - 804
SICI:
1431-6730(200105)382:5<799:ILOCSC>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIGEN PRESENTATION; DENDRITIC CELLS; PROTEOLYSIS; INHIBITORS; COMPLEX; DEGRADATION; PROTEINASES; EXPRESSION; PROTEASES; SELECTION;
Keywords:
antigen presentation; cathepsins; cysteine proteases; invariant chain; major histocompatibility complex class II;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Zavasnik-Bergant, V Jozef Stefan Inst, Dept Biochem & Mol Biol, Jamova 39,Ljubljana 1000, Slovenia Jozef Stefan Inst Jamova 39 Ljubljana Slovenia 1000 a
Citazione:
V. Zavasnik-Bergant et al., "Immunochemical localisation of cathepsin S, cathepsin L and MHC class II-associated p41 isoform of invariant chain in human lymph node tissue", BIOL CHEM, 382(5), 2001, pp. 799-804

Abstract

Antigen presentation by MHC class II molecules requires cysteine proteases(CP) for two convergent proteolytic processes: stepwise degradation of theinvariant chain (li) and generation of immunogenic peptides. Their activity is controlled by intracellular CP inhibitors, including presumably the p41 isoform of invariant chain (p41 li), which is in vitro a potent inhibitorof cathepsin L but not of cathepsin S. In order to evaluate the inhibitorypotential of p41 ii in antigen-presenting cells (APC), these three proteins were stained in lymph node tissue using specific monoclonal and polyclonal antibodies. The most abundant labelling was observed in subcapsular (cortical) and trabecular sinuses of the lymph node. In this area the most frequent APC were macrophages, as confirmed by the CD68 cell marker. Using confocal fluorescence microscopy, co-localisation of p41 li with cathepsin S, but not with cathepsin L was found in these cells. Our results are consistentwith the hypothesis that cathepsin S participates in degradation of the invariant chain, but they do not support the association between cathepsin L and p41 li in APC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 04:45:49