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Titolo:
Inducible nitric oxide production is an adaptation to cardiopulmonary bypass-induced inflammatory response
Autore:
Hayashi, Y; Sawa, Y; Fukuyama, N; Nakazawa, H; Matsuda, H;
Indirizzi:
Osaka Univ, Grad Sch Med, Course Intervent Med E1, Dept Surg, Suita, Osaka5650871, Japan Osaka Univ Suita Osaka Japan 5650871 ept Surg, Suita, Osaka5650871, Japan Tokai Univ, Sch Med, Dept Physiol 2, Kanagawa 2591100, Japan Tokai Univ Kanagawa Japan 2591100 ept Physiol 2, Kanagawa 2591100, Japan
Titolo Testata:
ANNALS OF THORACIC SURGERY
fascicolo: 1, volume: 72, anno: 2001,
pagine: 149 - 155
SICI:
0003-4975(200107)72:1<149:INOPIA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VIVO; PEROXYNITRITE PRODUCTION; CONTRACTILE DYSFUNCTION; LUNG INJURY; SYNTHASE; RAT; MACROPHAGES; SUBSTANCES; INHIBITION; INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Sawa, Y Osaka Univ, Grad Sch Med, Course Intervent Med E1, Dept Surg, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita OsakaJapan 5650871 5650871, Japan
Citazione:
Y. Hayashi et al., "Inducible nitric oxide production is an adaptation to cardiopulmonary bypass-induced inflammatory response", ANN THORAC, 72(1), 2001, pp. 149-155

Abstract

Background. Cardiopulmonary bypass (CPB) increases nitric oxide (NO) production by the activation of NO synthases (NOS). However, the role of NO frominducible NOS (iNOS) in CPB-induced inflammatory response remains unclear. We examined the effect of a selective iNOS inhibitor, aminoguanidine, on CPB-induced inflammatory response in a rat-CPB model. Methods. Adult Sprague-Dawley rats underwent 60 minutes of CPB (100 mL (.)kg(-1 .) min(-1), 34 degreesC). Group A (n = 10) received 100 mg/kg of aminoguanidine intraperitoneally 30 minutes before the initiation of CFB, and group B (n = 10) served as controls. Results. There were significant time-dependent changes in plasma interleukin (IL)-6, IL-8, nitrate + nitrite, the percentage ratio of nitrotyrosine to tyrosine (%NO2- Tyr, an indicator of peroxynitrite formation), and respiratory index (RI). Three hours after CPB termination, IL-6, IL-8, and RI were significantly higher in group A (IL-6, 397.5 +/- 80.6 pg/mL; IL-8, 26.99 /- 6.57 ng/mL; RI, 1.87 +/- 0.31) than in group B (IL-6, 316.5 +/- 73.9 pg/mL, p <0.05; IL-8, 17.21 +/- 3.12 ng/mL, p < 0.01; RI, 1.57 +/- 0.24, p < 0.05) although nitrate + nitrite (31.8 +/- 4.1 mu mol/L) and %NO2-Tyr (1.15% +/- 0.20%) were significantly lower in group A than in group B (nitrate nitrite, 50.2 +/- 5.0 mu mol/L, p < 0.01; %NO2-Tyr, 1.46% +/- 0.21%, p < 0.01). Western immunoblot analysis from lung tissue of group A identified marked iNOS inhibition without inhibiting endothelial-constitutive NOS, and neutrophil accumulation in the lung specimens was significantly greater in group A (6.5 +/- 0.7/alveoli) than in group B (4.4 +/- 0.4/alveoli, p < 0.01). Conclusions. These results suggest that NO production from iNOS may be an adaptive response for attenuating the CPB-induced inflammatory response. (C) 2001 by The Society of Thoracic Surgeons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 01:36:20