Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Activation of poly(ADP-ribose) polymerase in severe hemorrhagic shock and resuscitation
Autore:
Watts, JA; Grattan, RM; Whitlow, BS; Kline, JA;
Indirizzi:
Carolinas Med Ctr, Charlotte, NC 28232 USA Carolinas Med Ctr Charlotte NCUSA 28232 Med Ctr, Charlotte, NC 28232 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 2, volume: 281, anno: 2001,
pagine: G498 - G506
SICI:
0193-1857(200108)281:2<G498:AOPPIS>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADP-RIBOSE SYNTHETASE; CELLULAR-ENERGY DEPLETION; NITRIC-OXIDE SYNTHASE; DNA STRAND BREAKAGE; REPERFUSION INJURY; RAT MODEL; VASCULAR CONTRACTILE; GENERATES SUPEROXIDE; ORGAN INJURY; PEROXYNITRITE;
Keywords:
multiorgan failure; ileum; gut permeability; 3-aminobenzamide; caspase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Watts, JA Carolinas Med Ctr, POB 32861, Charlotte, NC 28232 USA Carolinas Med Ctr POB 32861 Charlotte NC USA 28232 NC 28232 USA
Citazione:
J.A. Watts et al., "Activation of poly(ADP-ribose) polymerase in severe hemorrhagic shock and resuscitation", AM J P-GAST, 281(2), 2001, pp. G498-G506

Abstract

This study examines activation of poly(ADP-ribose) polymerase (PARP) in the ileum during hemorrhage and resuscitation and determines if inhibition ofPARP reduces organ dysfunction and metabolic acidosis. Awake, nonheparinized rats were hemorrhaged (40 mmHg, 60 min). Resuscitation used Ringer's solution (2 1/3 x shed volume) and packed red blood cells (2/3 shed volume). Ileal PARP activity was elevated at the end of hemorrhage (3.6-fold) and 10 min of resuscitation (5-fold). The subsequent decline in PARP activity observed after 60 min of resuscitation was not due to cleavage by caspase-3. Ileum permeability increased 10-fold and circulating liver enzymes increased 4- to 6-fold following 60 min of resuscitation in animals pretreated with 3-amino-benzoic acid, a structural analog that does not inhibit PARP. Pretreatment with 3-aminobenzamide (3-AB), a PARP inhibitor, reduced these changes, whereas posttreatment with a bolus of 3-AB was ineffective. Metabolic acidosis, accumulation of lactate, and base deficit was reduced by pretreatment with 3-AB. PARP is activated in the ileum by hemorrhage and by resuscitation. Activation of PARP contributes to organ dysfunction in the ileum and liver and appears to be central to the development of metabolic acidosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 18:19:14