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Titolo:
Triptolide inhibits vascular endothelial growth factor expression and production in endothelial cells
Autore:
Hu, KB; Liu, ZH; Guo, XH; Liu, D; Li, LS;
Indirizzi:
Nanjing Univ, Sch Med, Res Inst Nephrol, Jinling Hosp, Nanjing 210002, Peoples R China Nanjing Univ Nanjing Peoples R China 210002 jing 210002, Peoples R China
Titolo Testata:
ACTA PHARMACOLOGICA SINICA
fascicolo: 7, volume: 22, anno: 2001,
pagine: 651 - 656
SICI:
0253-9756(200107)22:7<651:TIVEGF>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR MESSENGER-RNA; MINIMAL CHANGE; HUMAN KIDNEY; ANGIOGENESIS; RECEPTORS; LYMPHOCYTE; AP-1;
Keywords:
Tripterygium Wilfordii; triptolide; proteinuria; endothelial growth factors; vascular endothelium; proto-oncogene proteins c-jun;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, ZH Nanjing Univ, Sch Med, Res Inst Nephrol, Jinling Hosp, Nanjing 210002, Peoples R China Nanjing Univ Nanjing Peoples R China 210002 002, Peoples R China
Citazione:
K.B. Hu et al., "Triptolide inhibits vascular endothelial growth factor expression and production in endothelial cells", ACT PHAR SI, 22(7), 2001, pp. 651-656

Abstract

AIM: To investigate the effects of triptolide on vascular endothelial growth factor (VEGF) expression and secretion by endothelial cells, and explorethe mechanism of anti-proteinuric effect of triptolide on glomerulonephritis. METHODS: A human umbilical endothelium derived cell line (ECV-304) fromAmerican Type Culture Collection (ATCC) was used in this study. The effects of triptolide on VEGF mRNA expression, production, and secretion induced by 12- o-tetradecanoyl-phorbol-13-acetate (TPA) were measured by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), flow cytometry, and enzyme linked immunosorbent assay (ELISA) respectively. The endothelial c-fos/c-jun mRNA expression were also detected by RT-PCR after treatment of triptolide. RESULTS: VEGF mRNA expression was markedly up-regulated by TPA-stimulation. In addition, the production and secretion of VEGF in endothelial cells also increased in TPA treated cells. It was founded that triptolide inhibited VEGF mRNA expression, protein production and secretionin endothelial cells induced by TPA. Interestingly, TPA-induced c-fos/ c-jun mRNA expression in endothelial cells was also inhibited by triptolide. CONCLUSION: Triptolide is a potent inhibitor of VEGF expression and production ih endothelial cells. The inhibitory effects of triptolide on VEGF expression and production can contribute to its anti-proteinuric effect on glomerulonephritis. Downregulation of c-fos/c-jun expression in endothelial cells by triptolide is one of the mechanisms of the inhibitory effect of triptolide on VEGF expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 19:55:30