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Titolo:
IDENTIFICATION OF CIS-ACTING REPRESSOR ACTIVITY WITHIN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 PROTEASE SEQUENCES
Autore:
HUFFMAN KM; ARRIGO SJ;
Indirizzi:
MED UNIV S CAROLINA,DEPT MICROBIOL & IMMUNOL,BASIC SCI BLDG,ROOM 201,171 ASHLEY AVE CHARLESTON SC 29425 MED UNIV S CAROLINA,DEPT MICROBIOL & IMMUNOL CHARLESTON SC 29425
Titolo Testata:
Virology
fascicolo: 2, volume: 234, anno: 1997,
pagine: 253 - 260
SICI:
0042-6822(1997)234:2<253:IOCRAW>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LATE REPLACEMENT VECTOR; REV-RESPONSIVE ELEMENT; 3' UNTRANSLATED REGION; VIRAL MESSENGER-RNA; INHIBITORY SEQUENCES; NUCLEAR RETENTION; SPLICE SITES; ENV REGION; EXPRESSION; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
K.M. Huffman e S.J. Arrigo, "IDENTIFICATION OF CIS-ACTING REPRESSOR ACTIVITY WITHIN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 PROTEASE SEQUENCES", Virology, 234(2), 1997, pp. 253-260

Abstract

Human immunodeficiency virus type-1 (HIV-1) Rev overcomes negative elements within viral RNAs to allow expression of gag, pol, and env. Theeffect of Rev on protein and RNA expression of HIV-1 protease (PR)-containing constructs was investigated utilizing transient transfection of COS cells. Rev, through the Rev response element (RRE), resulted ina large increase in proteolytic activity and cytoplasmic RNA accumulation. Furthermore, Rev increased the level of total RNA produced by a PR-containing construct. The increase in cytoplasmic RNA accumulation in the presence of Rev indicated the presence of cis-acting repressor sequences (CRS) within the RNA produced by this construct, Therefore, components of the construct were analyzed for CRS activity. PR sequences in both sense and antisense orientations exhibited CRS activity. RRE sequences alone conferred a small CRS effect. Additional CRS activity was present within an unspliced RNA containing only nef and LTR sequences. These results indicate a novel form of cis-acting repressor activity within HIV-1 PR; this activity is exerted regardless of the orientation of PR and appears to function at the level of cytoplasmic or nuclear RNA stability. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:27:35