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Titolo:
Osteoclast-like cells in an in vitro model of bone destruction by rheumatoid synovium
Autore:
Suzuki, Y; Tsutsumi, Y; Nakagawa, M; Suzuki, H; Matsushita, K; Beppu, M; Aoki, H; Ichikawa, Y; Mizushima, Y;
Indirizzi:
St Marianna Univ, Sch Med, Dept Rheumatol, Miyamae Ku, Kawasaki, Kanagawa 2168512, Japan St Marianna Univ Kawasaki Kanagawa Japan 2168512 Kanagawa 2168512, Japan St Marianna Univ, Sch Med, Inst Med Sci, Miyamae Ku, Kawasaki, Kanagawa 2168512, Japan St Marianna Univ Kawasaki Kanagawa Japan 2168512 Kanagawa 2168512, Japan St Marianna Univ, Sch Med, Dept Orthoped Surg, Miyamae Ku, Kawasaki, Kanagawa 2168512, Japan St Marianna Univ Kawasaki Kanagawa Japan 2168512 Kanagawa 2168512, Japan Tokai Univ, Sch Med, Dept Pathol, Isehara, Kanagawa 25911, Japan Tokai Univ Isehara Kanagawa Japan 25911 l, Isehara, Kanagawa 25911, Japan
Titolo Testata:
RHEUMATOLOGY
fascicolo: 6, volume: 40, anno: 2001,
pagine: 673 - 682
SICI:
1462-0324(200106)40:6<673:OCIAIV>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING-FACTOR; CATHEPSIN-B; DIFFERENTIATION FACTOR; ARTHRITIS; RESORPTION; MACROPHAGES; TUMOR; SYNOVIOCYTES; FIBROBLAST; MONOCYTES;
Keywords:
rheumatoid arthritis; bone destruction; osteoclasts; synovial tissue macrophages; in vitro model;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Suzuki, Y St Marianna Univ, Sch Med, Dept Rheumatol, Miyamae Ku, 2-16-1 Sugao, Kawasaki, Kanagawa 2168512, Japan St Marianna Univ 2-16-1 Sugao Kawasaki Kanagawa Japan 2168512 an
Citazione:
Y. Suzuki et al., "Osteoclast-like cells in an in vitro model of bone destruction by rheumatoid synovium", RHEUMATOLOG, 40(6), 2001, pp. 673-682

Abstract

Objective. Osteoclasts may be involved in the process of rheumatoid bone destruction. To lest this hypothesis, we developed an in vitro model of bonedestruction by osteoclast-like cells derived from cultured rheumatoid synovial tissue without using any inducers. Methods. Synovial tissues were obtained from rheumatoid arthritis and osteoarthritis patients and tissue pieces of about 2 mm(3) that contained synovial lining were cultured. Multinucleated cells derived from cultured synovial tissues were studied cytochemically and morphologically for osteoclast-specific markers. Results. Fibroblast-like and macrophage-like cells from the tissue pieces proliferated in the coexistence of lymphocytes. After 14 days of culture, multinucleated cells with tartrate-resistant acid phosphatase activity appeared. These cells expressed vacuolar Hi-ATPase, the vitronectin receptor andcathepsin K. Although binding of I-125-labelled salmon calcitonin was verylow, the cells contained ringed structures of F-actin and showed strong bone-resorbing activity on ivory slices. Proliferation of macrophage-like cells and formation of multinucleated cells continued during 6 months of culture in the presence of fibroblast-like cells. The bone-resorbing activity ofmultinucleated cells derived from rheumatoid synovial tissue was much higher than that of cells from osteoarthritis synovial tissue, and was related to the disease activity of rheumatoid arthritis. Conclusion. Our culture system reproduced in vitro the process of bone destruction by rheumatoid synovium, including the proliferation and fusion of precursor cells, polarization, activation and bone tissue resorption. This system may provide a tool for understanding the mechanisms of bone destruction in rheumatoid arthritis and for the development of new therapies to prevent bone destruction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 09:44:37