Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Role of prostaglandins in the suppression of apoptosis in hen granulosa cells by transforming growth factor alpha
Autore:
Manchanda, R; Kim, JM; Tsang, BK;
Indirizzi:
Univ Ottawa, Ottawa Hosp, Ottawa Hlth Res Inst, Dept Obstet & Gynaecol,Reprod Biol Unit, Ottawa, ON K1Y 4E9, Canada Univ Ottawa Ottawa ON Canada K1Y4E9 iol Unit, Ottawa, ON K1Y 4E9, Canada Univ Ottawa, Ottawa Hosp, Ottawa Hlth Res Inst, Dept Cellular & Mol Med, Ottawa, ON K1Y 4E9, Canada Univ Ottawa Ottawa ON Canada K1Y 4E9 Mol Med, Ottawa, ON K1Y 4E9, Canada
Titolo Testata:
REPRODUCTION
fascicolo: 1, volume: 122, anno: 2001,
pagine: 91 - 101
SICI:
1470-1626(200107)122:1<91:ROPITS>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROLIFERATOR-ACTIVATED RECEPTORS; OVARIAN FOLLICULAR DEVELOPMENT; ADIPOCYTE DIFFERENTIATION; FOLLICLES; MECHANISMS; EXPRESSION; RAT; STIMULATION; INVOLVEMENT; SURVIVAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Tsang, BK Univ Ottawa, Ottawa Hosp, Ottawa Hlth Res Inst, Dept Obstet & Gynaecol,Reprod Biol Unit, Civ Campus, Ottawa, ON K1Y 4E9, Canada Univ OttawaCiv Campus Ottawa ON Canada K1Y 4E9 K1Y 4E9, Canada
Citazione:
R. Manchanda et al., "Role of prostaglandins in the suppression of apoptosis in hen granulosa cells by transforming growth factor alpha", REPRODUCT, 122(1), 2001, pp. 91-101

Abstract

Although transforming growth factor alpha (TGF-alpha) is known to be an important survival factor for granulosa cells, the cellular and molecular mechanisms involved are uncertain. The purpose of the present study was to investigate the possible involvement of prostaglandins in the anti-apoptotic action of TGF-alpha. Hen granulosa cells from healthy prehierarchical follicles (2-6 mm) cultured in serum-free medium underwent spontaneous apoptosis as demonstrated by DNA fragmentation and nuclear chromatin condensation. TGF-alpha (20 ng ml(-1)) stimulated maximum synthesis of prostaglandins (PGE and PGF) in granulosa cells and completely inhibited serum deprivation-induced apoptosis. The addition of an inhibitor of cyclooxygenase (COX; N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide (NS398) or ibuprofen) or phospholipase A(2) (PLA(2); aristolochic acid, 2-p-amylcinnamoyl amino-4-chlorobenzoic acid (ONO-RS-82) or arachidonyl triflouro methyl ketone (TFMK)), to the culture medium markedly suppressed the TGF-alpha -induced prostaglandinsynthesis and significantly increased granulosa cell apoptosis. The apoptotic effect of NS398 and aristolochic acid was completely inhibited by exogenous prostaglandins (PGF(2 alpha), PGE(1), PGE(2)) and arachidonic acid, respectively. However, exogenous prostaglandins failed to inhibit the PLA(2) inhibitor-induced apoptotic DNA fragmentation, implying that in addition toprostaglandins, arachidonic acid or leukotrienes may be important in transducing the anti-apoptotic action of TGF-alpha. In the absence of exogenous TGF-alpha, prostaglandins had no significant influence on granulosa cell apoptosis induced by serum withdrawal. These findings indicate that prostaglandin synthesis is a necessary, but not sufficient, event in the suppressionof granulosa cell apoptosis by TGF-alpha. Whether arachidonic acid or leukotrienes ave important in the anti-apoptotic action of TGF-alpha in hen granulosa cells remains to be determined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 09:43:20