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Titolo:
Towards defining the urinary proteome using liquid chromatography-tandem mass spectrometry - II. Limitations of complex mixture analyses
Autore:
Davis, MT; Spahr, CS; McGinley, MD; Robinson, JH; Bures, EJ; Beierle, J; Mort, J; Yu, W; Luethy, R; Patterson, SD;
Indirizzi:
Amgen Corp, Dept Biochem, Thousand Oaks, CA 91320 USA Amgen Corp ThousandOaks CA USA 91320 iochem, Thousand Oaks, CA 91320 USA Amgen Corp, Dept Genet, Thousand Oaks, CA 91320 USA Amgen Corp Thousand Oaks CA USA 91320 Genet, Thousand Oaks, CA 91320 USA Amgen Corp, Dept Computat Biol, Thousand Oaks, CA 91320 USA Amgen Corp Thousand Oaks CA USA 91320 t Biol, Thousand Oaks, CA 91320 USA
Titolo Testata:
PROTEOMICS
fascicolo: 1, volume: 1, anno: 2001,
pagine: 108 - 117
SICI:
1615-9853(200101)1:1<108:TDTUPU>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
GEL-SEPARATED PROTEINS; IDENTIFICATION; PEPTIDES; IONS;
Keywords:
liquid chromatography-mass spectrometry proteomics; urinary proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Patterson, SD Celera Genomics, 45 W Gude Dr, Rockville, MD 20850 USA Celera Genomics 45 W Gude Dr Rockville MD USA 20850 0850 USA
Citazione:
M.T. Davis et al., "Towards defining the urinary proteome using liquid chromatography-tandem mass spectrometry - II. Limitations of complex mixture analyses", PROTEOMICS, 1(1), 2001, pp. 108-117

Abstract

With an emphasis on obtaining a multitude of high quality tandem mass spectrometry spectra for protein identification, instrumental parameters are described for the liquid chromatography-tandem mass spectrometry analysis of trypsin digested unfractionated urine using a hybrid quadrupole-time-of-flight (Q-TOF) mass spectrometer. Precursor acquisition rates of up to 20 distinct precursors/minute in a single analysis were obtained through the use of parallel precursor selection (four precursors/survey period) and variablecollision induced dissociation integration time (1 to 6 periods summed). Maximal exploitation of the gas phase fractionated ions was obtained throughthe use of narrow survey scans and iterative data-dependent analyses incorporating dynamic exclusion. The impact on data fidelity as a product of data-dependent selection of precursor ions from a dynamically excluded field is discussed with regards to sample complexity, precursor selection rates, survey scan range and facile chemical modifications. Operational and post-analysis strategies are presented to restore data confidence and reconcile the greatest number of matched spectra.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 07:06:27