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Titolo:
CD28 stimulation regulates its association with N-ethylmaleimide-sensitivefusion protein and other proteins involved in vesicle sorting
Autore:
Heller, M; Watts, JD; Aebersold, R;
Indirizzi:
Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 ol Biotechnol, Seattle, WA 98195 USA
Titolo Testata:
PROTEOMICS
fascicolo: 1, volume: 1, anno: 2001,
pagine: 70 - 78
SICI:
1615-9853(200101)1:1<70:CSRIAW>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELL ACTIVATION; TANDEM MASS-SPECTROMETRY; TYROSINE PHOSPHORYLATION; MEMBRANE-FUSION; CYTOPLASMIC DOMAIN; RECEPTOR CD28; LIPID RAFTS; COSTIMULATION; TRANSPORT; NSF;
Keywords:
CD28; N-ethylmaleimide-sensitve fusion protein immunoprecipitation; celluar signaling; mass spectrometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Aebersold, R Inst Syst Biol, 4225 Roosevelt Way,NE Suite 200, Seattle, WA 98105 USA Inst Syst Biol 4225 Roosevelt Way,NE Suite 200 Seattle WA USA 98105
Citazione:
M. Heller et al., "CD28 stimulation regulates its association with N-ethylmaleimide-sensitivefusion protein and other proteins involved in vesicle sorting", PROTEOMICS, 1(1), 2001, pp. 70-78

Abstract

CD28 delivers a co-stimulatory signal for T cell antigen receptor induced activation of T cells through a mechanism which remains mostly elusive to date. In order to try and gain insight into CD28 function, we therefore applied state-of-the-art mass spectrometric protein identification technology to the analysis of CD28 immunoprecipitates prepared from Jurkat T cells. We found that N-ethylmaleimide-sensitive fusion protein (NSF) and other proteins with sequence similarities to proteins part of or implicated in vesicular protein sorting pathways, were associated with CD28 in a CD28 stimulation-dependent manner. Furthermore, N-ethylmaleimide treatment abolished the NSF/ CD28 interaction completely, and blocked CD28 association with a tyrosine phosphorylated 103 kDa protein in the activated cells. These results are suggestive of a potential model for CD28 co-stimulation regulated by an NSF-catalyzed mechanism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 23:49:39