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Titolo:
Ion intensity analysis of post-source decay fragmentation in curved-field reflectron matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of carbohydrates: For structural characterization of glycosylation in proteome analysis
Autore:
Yamagaki, T; Nakanishi, H;
Indirizzi:
Univ Tokyo, Dept Chem, Sch Sci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Sch Sci, Bunkyo Ku, Tokyo 1130033, Japan Natl Inst Biosci & Human Technol, Ibaraki, Osaka, Japan Natl Inst Biosci &Human Technol Ibaraki Osaka Japan araki, Osaka, Japan
Titolo Testata:
PROTEOMICS
fascicolo: 2, volume: 1, anno: 2001,
pagine: 329 - 339
SICI:
1615-9853(200102)1:2<329:IIAOPD>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
DESORPTION IONIZATION MALDI; GLYCOSIDIC LINKAGES; PRODUCT IONS; OLIGOSACCHARIDES; ISOMERS; DIFFERENTIATION; GALACTOSE; ADHESION; PEPTIDES; SPECTRA;
Keywords:
fragment; glycosylation; oligosaccharide; matrix-assisted laser desorption; ionization mass spectrometry; post-source decay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Yamagaki, T Univ Tokyo, Dept Chem, Sch Sci, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1130033 kyo 1130033, Japan
Citazione:
T. Yamagaki e H. Nakanishi, "Ion intensity analysis of post-source decay fragmentation in curved-field reflectron matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of carbohydrates: For structural characterization of glycosylation in proteome analysis", PROTEOMICS, 1(2), 2001, pp. 329-339

Abstract

The various kinds of oligosaccharides were analyzed by using post-source decay (PSD) fragmentation method of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Since the curved fieldreflectron MALDI-MS can record all fragment ions in a single measurement, we can discuss the fragment ion intensity in PSD mass spectrum more accurately. The intensities of the PSD fragment ions indicate the fine structure of the saccharide chains. The type of glycosyl linkages could be determined by the ion intensity analysis, and the stereo isomers of monosaccharides were distinguished by the MALDI-PSD fragment ion analysis. The linkage isomers and structural isomers were also distinguished by this method. The ion intensity analysis of curved-field reflectron MALDI-MS could be a powerful tool for glycosylation analysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:23:20