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Titolo:
Identification of protein-bound carbohydrates by mass spectrometry
Autore:
Harvey, DJ;
Indirizzi:
Oxford Glycobiol Inst, Dept Biochem, Oxford OX1 3QU, England Oxford Glycobiol Inst Oxford England OX1 3QU em, Oxford OX1 3QU, England
Titolo Testata:
PROTEOMICS
fascicolo: 2, volume: 1, anno: 2001,
pagine: 311 - 328
SICI:
1615-9853(200102)1:2<311:IOPCBM>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASSISTED-LASER-DESORPTION/IONIZATION; TIME-OF-FLIGHT; PERFORMANCE LIQUID-CHROMATOGRAPHY; COLLISION-INDUCED DISSOCIATION; N-LINKED OLIGOSACCHARIDES; DEFICIENT GLYCOPROTEIN SYNDROME; MAGNETIC-SECTOR INSTRUMENTS; COMPLEX OLIGOSACCHARIDES; NEUTRAL OLIGOSACCHARIDES; INDUCED FRAGMENTATION;
Keywords:
mass spectrometry; matrix-assisted laser desorption/ionization (MALDI); electrospray (ESI); carbohydrates; fragmentation; review;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
123
Recensione:
Indirizzi per estratti:
Indirizzo: Harvey, DJ Oxford Glycobiol Inst, Dept Biochem, S Parks Rd, Oxford OX1 3QU, England Oxford Glycobiol Inst S Parks Rd Oxford England OX1 3QU ngland
Citazione:
D.J. Harvey, "Identification of protein-bound carbohydrates by mass spectrometry", PROTEOMICS, 1(2), 2001, pp. 311-328

Abstract

This review outlines the use of modern methods of mass spectrometry, mainly based on electrospray ionization and matrix-assisted based desorption/ionization, for the identification of protein-bound carbohydrates. The techniques are briefly described together with methods for glycan release and purification prior to mass spectrometry. fragmentation of glycans, produced under various conditions, is described in the context of obtaining structural information at the sensitivity required for proteomic work.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 08:56:07