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Titolo:
A glycomic approach to the identification and characterization of glycoprotein function in cells transfected with glycosyltransferase genes
Autore:
Taniguchi, N; Ekuni, A; Ko, JH; Miyoshi, E; Ikeda, Y; Ihara, Y; Nishikawa, A; Honke, K; Takahashi, M;
Indirizzi:
Osaka Univ, Grad Sch Med, Dept Biochem, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 Biochem, Suita, Osaka 5650871, Japan
Titolo Testata:
PROTEOMICS
fascicolo: 2, volume: 1, anno: 2001,
pagine: 239 - 247
SICI:
1615-9853(200102)1:2<239:AGATTI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-ACETYLGLUCOSAMINYLTRANSFERASE-III; GAMMA-GLUTAMYL-TRANSPEPTIDASE; LECTIN AFFINITY ELECTROPHORESIS; ALPHA-1-6 FUCOSYL-TRANSFERASE; MAJOR SWINE XENOANTIGEN; HEPATITIS-B VIRUS; GDP-L-FUC; HEPATOCELLULAR-CARCINOMA; HEPATOMA-CELLS; GROWTH-FACTOR;
Keywords:
glycome; glycosyltransferase genes; alpha 1-6 fucosyltransferase; review;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Taniguchi, N Osaka Univ, Grad Sch Med, Dept Biochem, 2-2 Yamadaoka, Suita,Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 871, Japan
Citazione:
N. Taniguchi et al., "A glycomic approach to the identification and characterization of glycoprotein function in cells transfected with glycosyltransferase genes", PROTEOMICS, 1(2), 2001, pp. 239-247

Abstract

The transfection of glycoprotein glycosyltransferase genes into cells leads to modification of both the structure and function of the glycoproteins and as a result, changes in glycome patterns. N-glycan branching enzymes hold some promise as a model system for the identification of glycome patterns. Both N-acetylglucosaminyltransferase III and alpha1-6 fucosyltransferase are typical glycosyltransferases, which are involved in the branching of N-glycans. The resulting enzymatic products, bisecting N-GlcNAc and alpha1-6 fucose residues, are no longer modified by other glycosyltransferases and it is a relatively simple task to identify their modification by means of lectins. In this review, the glycome patterns of glycosyltransferase gene transfectants and the non-transfectants were compared by two-dimensional gel electrophoresis and lectin staining, and the biological significance of the two genes are described. Analyses of glycome patterns by transfecting glycosyltransferase genes will lead to new fields of study in the area of postgenome research.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:26:42