Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Phospho-proteomics: Evaluation of the use of enzymatic de-phosphorylation and differential mass spectrometric peptide mass mapping for site specific phosphorylation assignment in proteins separated by gel electrophoresis
Autore:
Larsen, MR; Sorensen, GL; Fey, SJ; Larsen, PM; Roepstorff, P;
Indirizzi:
Odense Univ, Univ So Denmark, Dept Biochem & Mol Biol, DK-5230 Odense M, Denmark Odense Univ Odense Denmark M ochem & Mol Biol, DK-5230 Odense M, Denmark Odense Univ, Univ So Denmark, Ctr Proteome Anal, DK-5230 Odense, Denmark Odense Univ Odense Denmark DK-5230 roteome Anal, DK-5230 Odense, Denmark
Titolo Testata:
PROTEOMICS
fascicolo: 2, volume: 1, anno: 2001,
pagine: 223 - 238
SICI:
1615-9853(200102)1:2<223:PEOTUO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSTTRANSLATIONAL MODIFICATIONS; SEQUENCE DATABASES; AFFINITY-CHROMATOGRAPHY; IDENTIFICATION; PHOSPHOPEPTIDES; DESORPTION; IONIZATION; MIXTURES; PURIFICATION; SUBSTRATE;
Keywords:
protein phosphorylation; 2-D gel electrophoresis; phospho-proteomics; matrix-assisted laser desorption/ionization mass spectrometry; differential peptide mass mapping; alkaline phosphatase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Roepstorff, P Odense Univ, Univ So Denmark, Dept Biochem & Mol Biol, Campusvej 55, DK-5230 Odense M, Denmark Odense Univ Campusvej 55 Odense DenmarkM Odense M, Denmark
Citazione:
M.R. Larsen et al., "Phospho-proteomics: Evaluation of the use of enzymatic de-phosphorylation and differential mass spectrometric peptide mass mapping for site specific phosphorylation assignment in proteins separated by gel electrophoresis", PROTEOMICS, 1(2), 2001, pp. 223-238

Abstract

Detection of phosphorylated proteins as well as assignment of the phosphorylated sites in such proteins is a major challenge in proteomics. In the present study we evaluate the use of enzymatic de-phosphorylation in combination with differential peptide mass mapping for identification of phosphorylated peptides in peptide mixtures derived from in-gel digested phospho-proteins. Phospho-peptides could be identified provided that improved sample preparation methods prior to mass spectrometric analysis were used. An attempt to identify the proteins visualized by [P-32] autoradiography in a proteomics study and their phosphorylation sites, demonstrated that protein identification was possible whereas reliable identification of the phospho-peptides requires more protein than normally available in our proteomics studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:47:49