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Titolo:
Use of antibodies for detection of phosphorylated proteins separated by two-dimensional gel electrophoresis
Autore:
Kaufmann, H; Bailey, JE; Fussenegger, M;
Indirizzi:
ETH Zurich, Inst Biotechnol, CH-8093 Zurich, Switzerland ETH Zurich Zurich Switzerland CH-8093 chnol, CH-8093 Zurich, Switzerland
Titolo Testata:
PROTEOMICS
fascicolo: 2, volume: 1, anno: 2001,
pagine: 194 - 199
SICI:
1615-9853(200102)1:2<194:UOAFDO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMOBILIZED PH GRADIENTS; 2-DIMENSIONAL ELECTROPHORESIS; CELL-CYCLE; MAP KINASE; MASS-SPECTROMETRY; ESCHERICHIA-COLI; HUMAN-BREAST; TYROSINE; ACTIVATION; CANCER;
Keywords:
phosphorylation; antibodies; phospho-proteome; review;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Fussenegger, M ETH Zurich, Inst Biotechnol, CH-8093 Zurich, Switzerland ETH Zurich Zurich Switzerland CH-8093 Zurich, Switzerland
Citazione:
H. Kaufmann et al., "Use of antibodies for detection of phosphorylated proteins separated by two-dimensional gel electrophoresis", PROTEOMICS, 1(2), 2001, pp. 194-199

Abstract

Protein phosphorylation and dephosphorylation are key regulatory mechanisms in prokaryotic and eukaryotic cells. Considering the role of phosphorylation in many human diseases, it appears a major challenge to refine on the methods to analyze the phospho-proteome. Here we review the use of monoclonal antibodies directed against specific phosphorylated amino acid residues to visualize phosphoproteins separated by two-dimensional gel electrophoresis. Strategies are described how this method can successfully be applied to create phospho-proteome maps of mammalian cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:31:02