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Titolo:
Differential expression of angiogenic cytokines by cell lines and primary cultures of human prostate cancer
Autore:
DallEra, MA; Shih, SJ; Yang, J; Benik, S; Gandour-Edwards, R; Evans, CP;
Indirizzi:
Univ Calif Davis, Sch Med, Dept Urol, Sacramento, CA 95817 USA Univ Calif Davis Sacramento CA USA 95817 t Urol, Sacramento, CA 95817 USA Univ Calif Davis, Sch Med, Dept Pathol, Sacramento, CA 95817 USA Univ Calif Davis Sacramento CA USA 95817 Pathol, Sacramento, CA 95817 USA
Titolo Testata:
PROSTATE CANCER AND PROSTATIC DISEASES
fascicolo: 2, volume: 4, anno: 2001,
pagine: 106 - 111
SICI:
1365-7852(2001)4:2<106:DEOACB>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; VASCULAR-PERMEABILITY FACTOR; FACTOR VEGF EXPRESSION; PLASMINOGEN-ACTIVATOR; TUMOR ANGIOGENESIS; DNA-SYNTHESIS; UROKINASE; MIGRATION; RECEPTOR; INDUCTION;
Keywords:
basic fibroblast growth factor; vascular endothelial growth factor; tumor necrosis factor alpha; urokinase; primary prostate culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Evans, CP Univ Calif Davis, Sch Med, Dept Urol, 4860 Y St,Suite 3500, Sacramento, CA95817 USA Univ Calif Davis 4860 Y St,Suite 3500 Sacramento CA USA95817 SA
Citazione:
M.A. Dall'Era et al., "Differential expression of angiogenic cytokines by cell lines and primary cultures of human prostate cancer", PROSTATE C, 4(2), 2001, pp. 106-111

Abstract

Studies on angiogenic cytokines usually are initially based upon their expression by available established cell lines. Our hypothesis is that established epithelial prostate cancer (CaP) cell lines do not accurately reflect angiogenic cytokine expression as compared to epithelial and stromal components of primary cultures generated from clinical CaP specimens. Serum free and growth factor free conditioned medium (CM) was collected from PC3, LNCaP, and their orthotopic selected prostate cancer sublines. Surgically acquired and pathologically confirmed neoplastic prostate tissue was selectivelygrown for selection of epithelial or stromal components, and CM was also collected. CM was assayed for urokinase (u-PA), basic fibroblast growth factor (bFCF), vascular endothelial growth factor (VEGF), and tumor necrosis factor alpha (TNF-ol). u-PA was expressed only by androgen independent cell lines, but was detectable in the epithelial and stromal cultures of androgensensitive primary cultures. bFGF was not secreted by cell lines nor epithelial primary cultures. VEGF was universally expressed, but TNF-alpha was not secreted by cells lines nor primary cultures. These data suggest that theexpression of angiogenic cytokines by established epithelial Cap cell lines does not reflect epithelial and stromal primary cultures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/06/20 alle ore 02:25:44