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Titolo:
Light-induced phase-delay of the chicken pineal circadian clock is associated with the induction of cE4bp4, a potential transcriptional repressor of cPer2 gene
Autore:
Doi, M; Nakajima, Y; Okano, T; Fukada, Y;
Indirizzi:
Univ Tokyo, Grad Sch Sci, Dept Biochem & Biophys, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Biophys, Bunkyo Ku, Tokyo 1130033, Japan CREST, JST, Tokyo 1130033, Japan CREST Tokyo Japan 1130033CREST, JST, Tokyo 1130033, Japan
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 14, volume: 98, anno: 2001,
pagine: 8089 - 8094
SICI:
0027-8424(20010703)98:14<8089:LPOTCP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRO-B LYMPHOCYTES; PHOTIC REGULATION; DROSOPHILA CLOCK; PERIOD HOMOLOGS; FIRING RHYTHMS; MECHANISM; CELLS; ENTRAINMENT; EXPRESSION; OSCILLATOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Fukada, Y Univ Tokyo, Grad Sch Sci, Dept Biochem & Biophys, Bunkyo Ku, Hongo 7-3-1, Tokyo 1130033, Japan Univ Tokyo Hongo 7-3-1 Tokyo Japan 1130033 Tokyo 1130033, Japan
Citazione:
M. Doi et al., "Light-induced phase-delay of the chicken pineal circadian clock is associated with the induction of cE4bp4, a potential transcriptional repressor of cPer2 gene", P NAS US, 98(14), 2001, pp. 8089-8094

Abstract

The chicken pineal gland contains the autonomous circadian oscillator together with the photic-input pathway. We searched for chicken pineal genes that are induced by light in a time-of-day-dependent manner, and isolated chicken homolog of bZIP transcription factor E4bp4 (cE4bp4) showing high similarity to vrille, one of the Drosophila clock genes. cE4bp4 was expressed rhythmically in the pineal gland with a peak at very early (subjective) nightunder both 12-h light/12-h dark cycle and constant dark conditions, and the phase was nearly opposite to the expression rhythm of cPer2, a chicken pineal clock gene. Luciferase reporter gene assays showed that cE4BP4 represses cPer2 promoter through a E4BP4-recognition sequence present in the 5 ' -flanking region, indicating that cE4BP4 can down-regulate the chick pineal cPer2 expression. In vivo light-perturbation studies showed that the prolongation of the light period to early subjective night maintained the high level expression of the pineal cE4bp4, and presumably as a consequence delayed the onset of the induction of the pineal cPer2 expression in the next morning. These light-dependent changes in the mRNA levels of the pineal cE46p4and cPer2 were followed by a phase-delay of the subsequent cycles of cE4bp4/cPer2 expression, suggesting that cE4BP4 plays an important role in the phase-delaying process as a light-dependent suppressor of cPer2 gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 13:58:22