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Titolo:
Visualization of biochemical networks in living cells
Autore:
Remy, I; Michnick, SW;
Indirizzi:
Univ Montreal, Dept Biochim, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 im, Montreal, PQ H3C 3J7, Canada
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 14, volume: 98, anno: 2001,
pagine: 7678 - 7683
SICI:
0027-8424(20010703)98:14<7678:VOBNIL>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-PROTEIN INTERACTIONS; FACTOR 4E-BINDING PROTEIN-1; 70-KDA S6 KINASE; DIHYDROFOLATE-REDUCTASE; MAMMALIAN PROTEIN; TOR PROTEINS; PHOSPHORYLATION; RAPAMYCIN; ACTIVATION; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Michnick, SW Univ Montreal, Dept Biochim, CP 6128,Succursale Ctr Ville, Montreal, PQ H3C 3J7, Canada Univ Montreal CP 6128,Succursale Ctr Ville Montreal PQ Canada H3C 3J7
Citazione:
I. Remy e S.W. Michnick, "Visualization of biochemical networks in living cells", P NAS US, 98(14), 2001, pp. 7678-7683

Abstract

Functional annotation of novel genes can be achieved by detection of interactions of their encoded proteins with known proteins followed by assays tovalidate that the gene participates in a specific cellular function. We report an experimental strategy that allows for detection of protein interactions and functional assays with a single reporter system. Interactions among biochemical network component proteins are detected and probed with stimulators and inhibitors of the network. In addition, the cellular location ofthe interacting proteins is determined. We used this strategy to map a signal transduction network that controls initiation of translation in eukaryotes, We analyzed 35 different pairs of full-length proteins and identified 14 interactions, of which five have not been observed previously, suggesting that the organization of the pathway is more ramified and integrated thanpreviously shown. Our results demonstrate the feasibility of using this strategy in efforts of genomewide functional annotation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 13:37:55