Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Repression of O-methyltransferase genes in transgenic tobacco affects lignin synthesis and plant growth
Autore:
Pincon, G; Maury, S; Hoffmann, L; Geoffroy, P; Lapierre, C; Pollet, B; Legrand, M;
Indirizzi:
Univ Strasbourg 1, CNRS, Inst Biol Mol Plantes, F-67084 Strasbourg, FranceUniv Strasbourg 1 Strasbourg France F-67084 , F-67084 Strasbourg, France INRA, Chim Biol Lab, F-78850 Thiverval Grignon, France INRA Thiverval Grignon France F-78850 F-78850 Thiverval Grignon, France
Titolo Testata:
PHYTOCHEMISTRY
fascicolo: 7, volume: 57, anno: 2001,
pagine: 1167 - 1176
SICI:
0031-9422(200108)57:7<1167:ROOGIT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
CINNAMYL-ALCOHOL-DEHYDROGENASE; PHENYLALANINE AMMONIA-LYASE; DOWN-REGULATION; MOSAIC-VIRUS; CONIFERYL ALDEHYDE; COA REDUCTASE; BIOSYNTHESIS; EXPRESSION; SYRINGYL; POPLAR;
Keywords:
Nicotiana tabacum; solanaceae; tobacco; antisense RNA expression; lignin; phenylpropanoid metabolism; O-methyltransferases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Legrand, M Univ Strasbourg 1, CNRS, Inst Biol Mol Plantes, 12 Rue Gen Zimmer, F-67084Strasbourg, France Univ Strasbourg 1 12 Rue Gen Zimmer Strasbourg France F-67084
Citazione:
G. Pincon et al., "Repression of O-methyltransferase genes in transgenic tobacco affects lignin synthesis and plant growth", PHYTOCHEM, 57(7), 2001, pp. 1167-1176

Abstract

Among the different enzymatic steps leading to lignin biosynthesis: two methylation reactions introduce the methyl groups borne by guaiacyl (G) and syringyl (S) units. Tobacco possesses a complex system of methylation comprising three classes of CCoAOMTs (caffeoyl-CoA-O-methyltransferases) and two classes of COMTs (caffeic acid OMTs). Antisense plants transformed with theCCoAOMT sequence alone or fused to COMT I sequence have been produced and compared to ASCOMT I plants in order to study the specific role of each OMTisoform in lignin biosynthesis, plant development and resistance to pathogens. Tobacco plants strongly inhibited in OMT activities have been selectedand analyzed. Whereas antisense COMT I plants exhibited no visual phenotype, CCoAOMT repression was shown to strongly affect the development of both single and double transformants: a reduction of plant growth and the alteration of flower development were observed in the most inhibited plants. Lignin analysis performed by Klason and thioacidolysis methods, showed a decrease in the lignin quantity and changes in the lignin structure of ASCCoAOMT and ASCCoAOMT/ASCOMT I transgenics but not in ASCOMT I plants. Inhibition of COMT I in single as well as in double transformed tobacco was demonstrated to decrease S unit synthesis and to provoke the accumulation of 5-hydroxyguaiacyl lignin units. ASCCoAOMT/ASCOMT I tobacco was affected in lignin amount and composition, thus demonstrating additive effects of inhibition of both enzymes. The changes of lignin profiles and the phenotypical and molecular alterations observed in the different transgenic lines were particularly prominent at the later stages of plant development. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:07:05