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Titolo:
Enhanced expression of basement-membrane-type heparan sulfate proteoglycanin tumor fibro-myxoid stroma of intrahepatic cholangiocarcinoma
Autore:
Sabit, H; Tsuneyama, K; Shimonishi, T; Harada, K; Cheng, J; Ida, H; Saku, T; Saito, K; Nakanuma, Y;
Indirizzi:
Kanazawa Univ, Sch Med, Dept Pathol 2, Kanazawa, Ishikawa 9208640, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9208640 wa, Ishikawa 9208640, Japan Niigata Univ, Sch Dent, Dept Pathol, Niigata, Japan Niigata Univ NiigataJapan Univ, Sch Dent, Dept Pathol, Niigata, Japan Toyama City Hosp, Div Pathol & Lab Med, Toyama, Japan Toyama City Hosp Toyama Japan Hosp, Div Pathol & Lab Med, Toyama, Japan
Titolo Testata:
PATHOLOGY INTERNATIONAL
fascicolo: 4, volume: 51, anno: 2001,
pagine: 248 - 256
SICI:
1320-5463(200104)51:4<248:EEOBHS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOID CYSTIC CARCINOMA; HEPATIC STELLATE CELLS; HIGH-AFFINITY RECEPTOR; SALIVARY-GLAND ORIGIN; GROWTH-FACTOR; HUMAN LIVER; CANCER CELLS; PERLECAN; DIFFERENTIATION; MOLECULES;
Keywords:
basement membrane; cholangiocarcinoma; heparan sulfate proteoglycan; hepatocellular carcinoma; metastatic liver carcinoma; perlecan;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Nakanuma, Y Kanazawa Univ, Sch Med, Dept Pathol 2, Kanazawa, Ishikawa 9208640, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9208640 9208640, Japan
Citazione:
H. Sabit et al., "Enhanced expression of basement-membrane-type heparan sulfate proteoglycanin tumor fibro-myxoid stroma of intrahepatic cholangiocarcinoma", PATHOL INT, 51(4), 2001, pp. 248-256

Abstract

To investigate the molecular mechanism for enhanced fibrous stroma formation in intrahepatic cholangiocarcinoma (ICC), we surveyed the expression pattern of basement-membrane-type heparan sulfate proteoglycan (HSPG; also known as perlecan) at the core protein and the mRNA level in ICC as well as inother liver neoplasms and reactive hepatic diseases. Immunohistochemistry of paraffin-embedded liver sections with hyaluronidase pretreatment showed that HSPG was present in small amounts in normal liver around the bile ducts and the blood vessels within the portal area. There was no evident expression within the hepatic lobules, Intense immunoexpression of HSPG was seen in the tumor-specific fibro-myxoid stroma of ICC and metastatic liver cancer originating from the colon. However, tumor-specific stroma of hepatocellular carcinomas showed little or no expression of HSPG, At the mRNA level, signals for HSPG were found in tumor cells of cholangiocarcinoma and metastatic colonic carcinomas, and in myofibroblasts in the tumor fibro-myxoid-specific stroma, From immunoprecipitation and reverse transcription-polymerasechain reaction (RT-PCR) analyses, a cultured human intrahepatic cholangiocarcinoma cell line (CCKS1), was found to express high levels of HSPG core protein and mRNA, These findings suggest that biliary and metastatic colon carcinoma cells as well as stromal myofibroblasts have a potential for HSPG production. In order to investigate the growth, invasion and metastatic ability of ICC, further study of the 'self-made' stromal component of ICC may provide a new approach.

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Documento generato il 27/01/20 alle ore 01:32:07