Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Malondialdehyde modification of proteins in vitro is enhanced in the presence of acetaldehyde
Autore:
Mooradian, AD; Reinacher, D; Li, JP; Pinnas, JL;
Indirizzi:
St Louis Univ, Sch Med, Div Endocrinol Diabet & Met, Dept Internal Med, StLouis, MO 63110 USA St Louis Univ St Louis MO USA 63110 t Internal Med, StLouis, MO 63110 USA Univ Arizona, Coll Med, Dept Med, Tucson, AZ USA Univ Arizona Tucson AZ USA v Arizona, Coll Med, Dept Med, Tucson, AZ USA
Titolo Testata:
NUTRITION
fascicolo: 7-8, volume: 17, anno: 2001,
pagine: 619 - 622
SICI:
0899-9007(200107/08)17:7-8<619:MMOPIV>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CIRCULATING ANTIBODIES; RED WINE; ADDUCTS; ETHANOL; ANTIOXIDANTS; RABBITS; LIVER; RATS;
Keywords:
malondialdehyde; free radicals; lipid peroxidation; acetaldehyde; alcohol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Mooradian, AD St Louis Univ, Sch Med, Div Endocrinol Diabet & Met, Dept Internal Med, 3691 Rutger,Suite 101, St Louis, MO 63110 USA St Louis Univ 3691 Rutger,Suite 101 St Louis MO USA 63110 SA
Citazione:
A.D. Mooradian et al., "Malondialdehyde modification of proteins in vitro is enhanced in the presence of acetaldehyde", NUTRITION, 17(7-8), 2001, pp. 619-622

Abstract

test the hypothesis that the cardioprotective effect of alcohol is relatedto the inhibition of malondialdehyde (MDA) modification of proteins by acetaldehyde (AA), we studied the effect of AA on MDA modification of bovine serum albumin (BSA) in vitro. BSA was incubated simultaneously with a fixed concentration of MDA (70 mM) and different concentrations of AA (120, 60, 30, 10, or 0 mM) for 24 h at 37 degreesC. The MDA-modified or AA-modified BSA was quantitated with immunoblotting by using specific anti-MDA and specific anti-AA protein antisera, respectively. In another set of experiments, BSA was incubated sequentially, first with different concentrations of AA and then with 70 mM of MDA. In both incubation protocols, the presence of AA and AA modification of BSA enhanced MDA binding. These in vitro observations suggest that the putative cardioprotective effects of alcohol or wine cannot be ascribed to AA-mediated reduction in MDA protein formation, a possible biochemical pathway of accelerated atherosclerosis. (C) Elsevier ScienceInc. 2001.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 04:07:54