Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A comparison of intraperitoneal and oral gavage administration in comet assay in mouse eight organs
Autore:
Sekihashi, K; Sasaki, T; Yamamoto, A; Kawamura, K; Ikka, T; Tsuda, S; Sasaki, YF;
Indirizzi:
Hachinohe Natl Coll Technol, Fac Chem & Biol Engn, Lab Genotox, Hachinohe,Aomori 0391192, Japan Hachinohe Natl Coll Technol Hachinohe Aomori Japan 0391192 0391192, Japan Chem Cpds Co Ltd, Safety Res Inst, Kiyota Ku, Sapporo, Hokkaido 0040839, Japan Chem Cpds Co Ltd Sapporo Hokkaido Japan 0040839 , Hokkaido 0040839, Japan Iwate Univ, Fac Agr, Dept Vet Med, Lab Vet Publ Hlth, Morioka, Iwate 0208550, Japan Iwate Univ Morioka Iwate Japan 0208550 lth, Morioka, Iwate 0208550, Japan
Titolo Testata:
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
fascicolo: 1-2, volume: 493, anno: 2001,
pagine: 39 - 54
SICI:
1383-5718(20010627)493:1-2<39:ACOIAO>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL GEL-ELECTROPHORESIS; DNA-DAMAGE; GENOTOXICITY; BENZOPYRENE; MICE; IARC; AZO;
Keywords:
mouse multiple organs; comet assay; alkaline single cell gel electrophoresis (SCG) assay; route of administration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Sasaki, YF Hachinohe Natl Coll Technol, Fac Chem & Biol Engn, Lab Genotox,Tamonoki Uwanotai 16-1, Hachinohe, Aomori 0391192, Japan Hachinohe Natl Coll Technol Tamonoki Uwanotai 16-1 Hachinohe Aomori Japan 0391192
Citazione:
K. Sekihashi et al., "A comparison of intraperitoneal and oral gavage administration in comet assay in mouse eight organs", MUT RES-GTE, 493(1-2), 2001, pp. 39-54

Abstract

One of the important advantages of the comet assay is its ability to detect genotoxicity in many different organs. Since the exposure route of the test compounds is likely to influence the genotoxicity detected in a given organ, it is an important factor to consider when conducting the assay. In this study, we compared the effects of numerous model compounds on eight organs when administered to mice by intraperitoneal (i.p.) injection and oral (p.o.) gavage. Groups of four mice were treated once i.p. or p.o. at the identical proportion of LD50 for each route, and the stomach, colon, liver, kidney, bladder, lung, brain, and bone marrow were sampled 3, 8, and 24h after treatment. For 19 of the 20 tested mutagens with various modes of action, genotoxicityin some organs varied with treatment route; only the genotoxicity of methyl methane sulfonate was not affected. Treatment route, however, did not produce a qualitative difference in the genotoxicity of promutagens at the sites of conversion to ultimate mutagens, with aromatic hydrocarbons as the exception. When chemicals with positive responses in at least one organ were considered to be comet assay-positive, the administration route made no difference. Since azo reduction is mediated by azo reductase synthesized in the gastrointestinal wall and by gut microflora and i,p.-administered azo dyes bypass their activation site (colon), the administration route is expected to make a difference in their in vivo genotoxicity. Direct-acting mutagens are expected to affect the mucosa of the gastrointestinal tract when given p.o. For those mutagens, however, the administration route did not make aqualitative difference in gastrointestinal tract genotoxicity. Moreover, although the gastrointestinal mucosa is the first site to be exposed to p.o.administered agents, the peak times in the stomach tended to be the same as in most other organs. Based on those results, we concluded that the genotoxicity at high exposures was due to a systemic effect, and that both routes are acceptable for the comet assay when the liver and gastrointestinal organs are sampled, so long as appropriate dose levels for systemic exposure are selected for each route. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 18:05:23