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Titolo:
Characterization of O-6-methylguanine-DNA-methyltransferase (O-6-MGMT) activity in Xiphophorus fishes
Autore:
Walter, RB; Sung, HM; Intano, GW; Walter, CA;
Indirizzi:
SW Texas State Univ, Dept Chem & Biochem, San Marcos, TX 78666 USA SW Texas State Univ San Marcos TX USA 78666 hem, San Marcos, TX 78666 USA Univ Texas, Hlth Sci Ctr, Dept Cellular & Struct Biol, San Antonio, TX 78284 USA Univ Texas San Antonio TX USA 78284 truct Biol, San Antonio, TX 78284 USA
Titolo Testata:
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
fascicolo: 1-2, volume: 493, anno: 2001,
pagine: 11 - 22
SICI:
1383-5718(20010627)493:1-2<11:COO(A>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE ACTIVITY; METHYL-N-NITROSOUREA; ESCHERICHIA-COLI; ALKYLATING-AGENTS; DNA-GLYCOSYLASES; INDUCED MELANOMA; GENETIC BASIS; X-RAYS; METHYLTRANSFERASE; REPAIR;
Keywords:
mutagenesis; alkylation; DNA repair; O-6-methylguanine; Xiphophorus; fish;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Walter, RB SW Texas State Univ, Dept Chem & Biochem, 419 Centennial Hall,601 Univ Dr,San Marcos, TX 78666 USA SW Texas State Univ 419 Centennial Hall,601 Univ Dr San Marcos TX USA 78666
Citazione:
R.B. Walter et al., "Characterization of O-6-methylguanine-DNA-methyltransferase (O-6-MGMT) activity in Xiphophorus fishes", MUT RES-GTE, 493(1-2), 2001, pp. 11-22

Abstract

We utilized a custom-synthesized double-strand oligonucleotide containing a single O-6-methylguanine (O-6-MG) residue within a restriction endonuclease recognition site to determine O-6-methylguanine-DNA-methyltransferase (O-6-MGMT) activity in various tissue extracts prepared from Xiphophorus fish. The results suggest Xiphophorus fish O-6-MGMT activity has many of the same characteristics as Escherichia coli and mammalian O-6-MGMT's including rapid reaction kinetics consistent with stoichiometric removal of methyl groups, but exhibits a temperature optimum of 23 degreesC. Results from protein extract activity assays indicate O-6-MGMT activity patterns among four Xiphophorus tissues followed the order: brain greater than or equal to testes > gill greater than or equal to liver. In mammals, O-6-MGMT activity is high in liver, while activity in brain is minimal (i.e. approximate to9% of liver); however, we report that in the Xiphophorus fishes examined, brain tissue extracts exhibited much higher (approximate to six-fold) O-6-MGMT activity levels than liver. Comparison of O-6-MGMT activitybetween Xiphophorus species employed in tumor induction experiments did not indicate significant differences in ability to clear the pre-mutagenic O-6-MG from the oligonucleotide substrate. (C) 2001 Elsevier Science B.V. Allrights reserved.

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Documento generato il 10/07/20 alle ore 14:54:09