Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Nucleotide excision repair gene expression in the rat conceptus during organogenesis
Autore:
Vinson, RK; Hales, BF;
Indirizzi:
McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ H3G 1Y6, Canada McGill Univ Montreal PQ Canada H3G 1Y6 peut, Montreal, PQ H3G 1Y6, Canada
Titolo Testata:
MUTATION RESEARCH-DNA REPAIR
fascicolo: 2, volume: 486, anno: 2001,
pagine: 113 - 123
SICI:
0921-8777(20010712)486:2<113:NERGEI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA LEVELS; DNA-REPAIR; XERODERMA-PIGMENTOSUM; MOLECULAR MECHANISM; MOUSE EMBRYOS; CELL-LINES; CYCLOPHOSPHAMIDE; DAMAGE; SENSITIVITY; MUTATIONS;
Keywords:
nucleotide excision repair; DNA repair; rat conceptus; whole embryo culture; teratogen; 4-hydroperoxycyclophosphamide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Hales, BF McGill Univ, Dept Pharmacol & Therapeut, 3655 Promenade Sir William Osler,Montreal, PQ H3G 1Y6, Canada McGill Univ 3655 Promenade Sir William Osler Montreal PQ Canada H3G 1Y6
Citazione:
R.K. Vinson e B.F. Hales, "Nucleotide excision repair gene expression in the rat conceptus during organogenesis", MUT R-DNA R, 486(2), 2001, pp. 113-123

Abstract

DNA repair may be a determinant of the susceptibility of the conceptus to DNA damaging teratogens. The nucleotide excision repair (NER) pathway repairs a substantial amount of chemically induced DNA damage. The goals of thisstudy were to assess the coordinate expression of NER genes in the midorganogenesis-stage rat conceptus and determine the consequences of exposure tothe genotoxic teratogen, 4-hydroperoxycyclophosphamide (4-OOHCPA), on NER gene expression. Most NER genes were expressed at low levels in both yolk sac and embryo on gestational day (GD) 10, with the exception of XPD, XPE and PCNA. No significant alterations in gene expression occurred between GDs 10 and 11; in the yolk sac XPB expression increased on GD12, compared to either GD10 or 11. In the embryo, XPE expression increased between GDs 10 and12, while hHR23B, XPB, ERCC1, and DNA polymerase E expression increased onGD12 relative to both GDs 10 and 11. Contrary to gene expression data, XPBprotein was found at high levels and XPD at low levels in GDs 10-12 embryos and yolk sacs. Mirroring gene expression, high levels of PCNA protein were found in both tissues; XPA protein levels were minimal in yolk sac from GDs 10-12 but increased in the embryo from moderate on GD10 to high on GD12. Therefore, NER gene expression during organogenesis was regulated in a developmental stage- and tissue-specific manner. Exposure of the conceptus to a teratogen, 4-OOHCPA, induced malformations without affecting NER transcript levels. Thus, NER gene expression in the conceptus was unresponsive to regulation by DNA alkylation. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 09:01:12