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Titolo:
Subretinal injections in rodent eyes: effects on electrophysiology and histology of rat retina
Autore:
Timmers, A; Zhang, H; Squitieri, A; Gonzalez-Pola, C;
Indirizzi:
Univ Florida, Dept Ophthalmol, Gainesville, FL 32610 USA Univ Florida Gainesville FL USA 32610 hthalmol, Gainesville, FL 32610 USA
Titolo Testata:
MOLECULAR VISION
fascicolo: 19, volume: 7, anno: 2001,
pagine: 131 - 137
SICI:
1090-0535(20010622)7:19<131:SIIREE>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE-TRANSFER; RECOMBINANT ADENOASSOCIATED VIRUS; CILIARY NEUROTROPHIC FACTOR; PHOTORECEPTOR DEGENERATION; PIGMENT-EPITHELIUM; IN-VIVO; MOUSE RETINA; RD MOUSE; EXPRESSION; THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Timmers, A Univ Florida, Dept Ophthalmol, Box 100284 JHMHSC,Room D4-38D, Gainesville,FL 32610 USA Univ Florida Box 100284 JHMHSC,Room D4-38D Gainesville FL USA 32610
Citazione:
A. Timmers et al., "Subretinal injections in rodent eyes: effects on electrophysiology and histology of rat retina", MOL VIS, 7(19), 2001, pp. 131-137

Abstract

PURPOSE: To describe a reliable and fast method for subretinal injection in rodents and to assess the effect of the procedure on retinal function andhistology. METHODS: Corneas of rodents were punctured with a 28 gauge hypodermic insulin needle avoiding the lens. The injection procedure can be observed with the aid of a dissecting microscope and methylcellulose solution on the eye. A 33 gauge blunt needle was inserted into the eye through the corneal puncture and guided toward the subretinal matrix. Addition of fluorescein to the injection mixture facilitated immediate evaluation of the injection. Rat eyes were either non-injected (controls), received only a corneal puncture or were injected with fluorescent microspheres or PBS-fluorescein mixture. Retinal function and integrity were assessed through electroretinographic (ERG) analysis and postmortem histology. RESULTS: The anterior injection procedure provided a fast and simple method for subretinal injections. In rats a successful subretinal delivery was achieved in more than 90%, with less than 5% of the injected eyes developingcataracts. No significant differences in b-wave ERG amplitudes in rodent eyes over a five-week period were observed between non-injected control eyesand subretinally injected eyes (1 to 10 mul of PBS-fluorescein or 2 mul fluorescent microspheres). Histological analysis revealed that re-attachment of the rat retina occurred in approximately 1 day post-injection and the phagocytotic ability of RPE cells remained intact. CONCLUSIONS: This method was easily learned and required a minimum of equipment and animal preparation. With experience, 10 to 30 eyes could be injected per h. Furthermore, the injection procedure did not compromise the lens, retina or retinal pigment epithelium (RPE).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 22:11:31