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Titolo:
Localization of pigment epithelium derived factor (PEDF) in developing andadult human ocular tissues
Autore:
Karakousis, PC; John, SK; Behling, KC; Surace, EM; Smith, JE; Hendrickson, A; Tang, WX; Bennett, J; Milam, AH;
Indirizzi:
Univ Penn, Scheie Eye Inst, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Eye Inst, Philadelphia, PA 19104 USA Univ Penn, FM Kirby Ctr Mol Ophthalmol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 phthalmol, Philadelphia, PA 19104 USA Univ Washington, Dept Biol Struct, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 t Biol Struct, Seattle, WA 98195 USA
Titolo Testata:
MOLECULAR VISION
fascicolo: 22, volume: 7, anno: 2001,
pagine: 154 - 163
SICI:
1090-0535(20010630)7:22<154:LOPEDF>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBELLAR GRANULE CELLS; NEUROTROPHIC ACTIVITY; RETINOBLASTOMA CELLS; NONINHIBITORY SERPIN; RETINITIS-PIGMENTOSA; MOTOR-NEURONS; GROWTH-FACTOR; EXPRESSION; GENE; DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Milam, AH Univ Penn, Scheie Eye Inst, 51 N 39th St, Philadelphia, PA 19104USA Univ Penn 51 N 39th St Philadelphia PA USA 19104 a, PA 19104 USA
Citazione:
P.C. Karakousis et al., "Localization of pigment epithelium derived factor (PEDF) in developing andadult human ocular tissues", MOL VIS, 7(22), 2001, pp. 154-163

Abstract

PURPOSE: To localize pigment epithelium-derived factor (PEDF) in developing and adult human ocular tissues. METHODS: PEDF was localized in fetal and adult eyes by immunofluorescence with a polyclonal antibody (pAb) against amino acids 327-343 of PEDF, or a monoclonal antibody (mAb) against the C-terminal 155 amino acids of PEDF. Specificity of the antibodies was documented by Western blotting. PEDF mRNA was localized in adult retina by in situ hybridization. RESULTS: In developing retinas (7.4 to 21.5 fetal weeks, Fwks), pAb anti-PEDF labeled retinal pigment epithelium (RPE) granules, developing cones, some neuroblasts and many cells in the ganglion cell layer (GCL). In adult retinas, pAb anti-PEDF labeled rod and cone cytoplasm and nuclei of rods but not cones. Cells in the INL and GCL, choroid, corneal epithelium and endothelium, and ciliary body were also pAb PEDF-positive. Preadsorption of pAb anti-PEDF with the immunizing peptide blocked specific labeling in retina and other tissues, except for photoreceptor outer segments. In agreement withthe immunolocalization with pAb anti-PEDF, in situ hybridization revealed PEDF mRNA in the RPE, photoreceptors, inner nuclear layer cells and ganglion cells in adult retina. In developing retinas 18 Fwks and older, and in adult retinas, mAb anti-PEDF labeled the interphotoreceptor matrix (IPM). Western blots of retina, cornea, and ciliary body/iris with pAb anti-PEDF produced several bands at about 46 kDa. With mAb anti-PEDF, retina produced oneband at about 46 kDa; cornea and ciliary body/iris had several bands at about 46 kDa. CONCLUSIONS: PEDF, originally reported as a product of RPE cells, is present in photoreceptors and inner retinal cell types in developing and adult human eyes. Photoreceptors and RPE may secrete PEDF into the IPM.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 03:17:59