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Titolo:
Apoptosis in the normal human amnion at term, independent of Bcl-2 regulation and onset of labour
Autore:
Kumagai, K; Otsuki, Y; Ito, Y; Shibata, MA; Abe, H; Ueki, M;
Indirizzi:
Osaka Med Coll, Dept Obstet & Gynecol, Takatsuki, Osaka 5698686, Japan Osaka Med Coll Takatsuki Osaka Japan 5698686 atsuki, Osaka 5698686, Japan
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 7, volume: 7, anno: 2001,
pagine: 681 - 689
SICI:
1360-9947(200107)7:7<681:AITNHA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN UTERINE ENDOMETRIUM; HUMAN FETAL MEMBRANES; DNA STRAND BREAKS; MENSTRUAL-CYCLE; FAS LIGAND; HUMAN PLACENTA; MATRIX METALLOPROTEINASE-9; VILLOUS CYTOTROPHOBLAST; EXTRACELLULAR-MATRIX; HUMAN TROPHOBLAST;
Keywords:
amnion; apoptosis; caspase; Fas antigen; Fas ligand;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Otsuki, Y Osaka Med Coll, Dept Obstet & Gynecol, 2-7 Daigaku Machi, Takatsuki, Osaka5698686, Japan Osaka Med Coll 2-7 Daigaku Machi Takatsuki Osaka Japan 5698686 n
Citazione:
K. Kumagai et al., "Apoptosis in the normal human amnion at term, independent of Bcl-2 regulation and onset of labour", MOL HUM REP, 7(7), 2001, pp. 681-689

Abstract

This study was designed to detect apoptosis in the human amnion and to elucidate the signalling pathway involved in its regulation, Samples of human amnion were obtained from 34 women (weeks 11-42 of gestation) and studied using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) method with light microscopy (LM) and transmission electron microscopy (TEM), Apoptotic regulators in the samples were studied by immunohistochemistry and caspase activity assay. The TUNEL method with LM demonstrated that the percentage of TUNEL-positive cells in the amniotic epithelium was the highest in weeks 40-41 of gestation (P < 0.05) independent of the onset of labour, and the cells were often detached from the epithelium into the amniotic cavity at term, The TUNEL method with TEM clearly showed the characteristic features of apoptosis such as the nuclear condensed chromatin with abundant free 3 ' -OH DNA ends, cell shrinkage and a decreasein the number of desmosomes, except for the presence of apoptotic bodies, Fas and Fas ligand (FasL) were constantly expressed on apical membranes of amniotic epithelial cells from weeks 16-27 through to 40-41 of gestation, while no Bcl-2 expression was observed throughout the gestational periods. Activities of caspase-3 and caspase-8, but not of caspase-9, were higher in weeks 40-41 than those from weeks 16-27 of gestation (P < 0.01). We conclude that apoptosis in term amniotic epithelium is independent of Bcl-2 regulation and onset of labour, and may play an important role in the fragility and rupture of human fetal membranes at term.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 14:56:12