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Titolo:
Changes of the major sperm maturation-associated epididymal protein HE5 (CD52) on human ejaculated spermatozoa during incubation in capacitation conditions
Autore:
Yeung, CH; Perez-Sanchez, F; Schroter, S; Krichhoff, C; Cooper, TG;
Indirizzi:
Univ Munster, Inst Reprod Med, D-4400 Munster, Germany Univ Munster Munster Germany D-4400 Reprod Med, D-4400 Munster, Germany Univ Valencia, Dept Cell Biol, Valencia, Spain Univ Valencia Valencia Spain Valencia, Dept Cell Biol, Valencia, Spain Inst Hormone & Fertil Res, D-2000 Hamburg, Germany Inst Hormone & Fertil Res Hamburg Germany D-2000 D-2000 Hamburg, Germany
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 7, volume: 7, anno: 2001,
pagine: 617 - 624
SICI:
1360-9947(200107)7:7<617:COTMSM>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOSYLPHOSPHATIDYLINOSITOL-ANCHORED GLYCOPROTEIN; 20 KDA SIALYLGLYCOPROTEIN; HUMAN SEMINAL PLASMA; LIPID ARCHITECTURE; QUALITY-CONTROL; BOAR SPERM; ANTIGEN; ANTIBODY; CHOLESTEROL; MEMBRANES;
Keywords:
cryptic epitope; epididymal secretion; flow cytometry; sperm protein; surface antigen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Yeung, CH Univ Munster, Inst Reprod Med, D-4400 Munster, Germany Univ Munster Munster Germany D-4400 d, D-4400 Munster, Germany
Citazione:
C.H. Yeung et al., "Changes of the major sperm maturation-associated epididymal protein HE5 (CD52) on human ejaculated spermatozoa during incubation in capacitation conditions", MOL HUM REP, 7(7), 2001, pp. 617-624

Abstract

HE5 (CD52) is a glycoprotein which is secreted by the epididymis and whichbecomes inserted onto maturing spermatozoa. We have previously shown that,in cynomolgus monkey spermatozoa, changes occur upon maturation rendering cryptic the epitope to the monoclonal antibody CAMPATH-1G; the recognition site is then re-exposed during incubation under capacitation conditions, The present study investigated human ejaculated spermatozoa during incubationunder similar conditions, using monoclonal antibodies that recognize different epitopes of the HE5 molecule comprising parts of the N-glycan (2E5) orpeptide segments, including (CAMPATH-1G) or excluding (097) the glycosylphosphatidylinositol (GPI) anchor, to reveal modifications of sperm surface HE5, Flow cytometric analysis showed equally high percentages (similar to 90%) of viable spermatozoa cross-reacting with the antibodies before and after 6 h incubation. However, during incubation, the staining intensity increased 57% with CAMPATH-1G, 31% with 097, but remained unchanged with 2E5, Thelymphocyte CD52 antibody CF1D12 stained only similar to 10% of spermatozoaeither before or after incubation, Western blotting of sperm protein extracts using lectins indicated an increase in the exposure of sialic acid residues of HE5 after incubation. These results suggest that during incubation in capacitating conditions, there is an opening up of the HE5 glycoprotein molecule, increasing accessibility of some sialic acid residues and of the core peptide, particularly the GPI anchor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 21:11:13